Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord

Li Yen Mae Huang; Susan M. Carlton; William D. Willis

doi:10.1016/0165-0270(85)90119-0

Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord

Li Yen Mae Huang, Susan M. Carlton, William D. Willis

Neuroscience & Cell

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.

Original language	English (US)
Pages (from-to)	91-96
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/0165-0270(85)90119-0
State	Published - Jul 1985

Keywords

cell identification
diamidino yellow dihydrochloride
fluorescent label
fresh
rat spinal cord
retrograde marker
spinothalamic tract cell
unfixed spinal cord

ASJC Scopus subject areas

General Neuroscience

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10.1016/0165-0270(85)90119-0

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title = "Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord",

abstract = "Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.",

keywords = "cell identification, diamidino yellow dihydrochloride, fluorescent label, fresh, rat spinal cord, retrograde marker, spinothalamic tract cell, unfixed spinal cord",

author = "Huang, {Li Yen Mae} and Carlton, {Susan M.} and Willis, {William D.}",

year = "1985",

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language = "English (US)",

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journal = "Journal of Neuroscience Methods",

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T1 - Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord

AU - Huang, Li Yen Mae

AU - Carlton, Susan M.

AU - Willis, William D.

PY - 1985/7

Y1 - 1985/7

N2 - Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.

AB - Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.

KW - cell identification

KW - diamidino yellow dihydrochloride

KW - fluorescent label

KW - fresh

KW - rat spinal cord

KW - retrograde marker

KW - spinothalamic tract cell

KW - unfixed spinal cord

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JO - Journal of Neuroscience Methods

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ER -

Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord

Abstract

Keywords

ASJC Scopus subject areas

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