Identification of dynorphin 1-8 in human placenta by mass spectrometry

Mahmoud S. Ahmed; Laura W. Randall; Baha Sibai; Chhabil Dass; Genevieve Fridland; Dominic M. Desiderio; Emirgul Tolun

doi:10.1016/0024-3205(87)90099-3

Identification of dynorphin 1-8 in human placenta by mass spectrometry

Mahmoud S. Ahmed, Laura W. Randall, Baha Sibai, Chhabil Dass, Genevieve Fridland, Dominic M. Desiderio, Emirgul Tolun

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H)⁺ ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.

Original language	English (US)
Pages (from-to)	2067-2076
Number of pages	10
Journal	Life Sciences
Volume	40
Issue number	21
DOIs	https://doi.org/10.1016/0024-3205(87)90099-3
State	Published - May 25 1987
Externally published	Yes

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(87)90099-3

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title = "Identification of dynorphin 1-8 in human placenta by mass spectrometry",

abstract = "The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H)+ ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.",

author = "Ahmed, {Mahmoud S.} and Randall, {Laura W.} and Baha Sibai and Chhabil Dass and Genevieve Fridland and Desiderio, {Dominic M.} and Emirgul Tolun",

note = "Funding Information: This work has been supported in part by a National Science Foundation grant PCM 83-17217 to MSA and an NIH grant GM 26666 to DMD.",

year = "1987",

month = may,

day = "25",

doi = "10.1016/0024-3205(87)90099-3",

language = "English (US)",

volume = "40",

pages = "2067--2076",

journal = "Life Sciences",

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T1 - Identification of dynorphin 1-8 in human placenta by mass spectrometry

AU - Ahmed, Mahmoud S.

AU - Randall, Laura W.

AU - Sibai, Baha

AU - Dass, Chhabil

AU - Fridland, Genevieve

AU - Desiderio, Dominic M.

AU - Tolun, Emirgul

N1 - Funding Information: This work has been supported in part by a National Science Foundation grant PCM 83-17217 to MSA and an NIH grant GM 26666 to DMD.

PY - 1987/5/25

Y1 - 1987/5/25

N2 - The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H)+ ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.

AB - The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H)+ ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.

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Identification of dynorphin 1-8 in human placenta by mass spectrometry

Abstract

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