Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility

Benjamin D. Byers; Egle Bytautiene; Maged M. Costantine; Catalin S. Buhimschi; Irina Buhimschi; George R. Saade; Nima Goharkhay

doi:10.1016/j.ajog.2010.07.031

Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility

Benjamin D. Byers, Egle Bytautiene, Maged M. Costantine, Catalin S. Buhimschi, Irina Buhimschi, George R. Saade, Nima Goharkhay

Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

OBJECTIVE: To investigate the effect of intracervical hyaluronidase on the biomechanical properties of the cervix and on uterine contractility. STUDY DESIGN: Sprague-Dawley rats (n=33, term day 22) were injected with hyaluronidase (100 IU) or saline solution on day 8 of gestation (n=8-9/group). On day 21, labor was induced with mifepristone (8 mg/rat). Injection-to-delivery times were recorded. Biomechanical properties of the cervix were assessed using stretch-tension analysis. Myometrial contractility was investigated in response to hyaluronidase (0.2-200 IU/mL), oxytocin (10^-10M to 10 ^-5M), and potassium chloride (60 mM). RESULTS: Delivery times were shorter in the hyaluronidase group (P= .03). Cervices of the treated animals showed higher measures of elasticity and plasticity (P= .02 for both). Myometrial sensitivity to hyaluronidase, oxytocin, or potassium chloride was not affected by the cervical application of hyaluronidase (P>.05 for all). CONCLUSION: Cervical hyaluronidase treatment shortens labor and alters the biomechanical properties of the cervix, independent of the myometrium.

Original language	English (US)
Pages (from-to)	596.e1-596.e5
Journal	American journal of obstetrics and gynecology
Volume	203
Issue number	6
DOIs	https://doi.org/10.1016/j.ajog.2010.07.031
State	Published - Dec 2010

Keywords

Cervical ripening
Hyaluronidase
Labor
Myometrium

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2010.07.031

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Byers, B. D., Bytautiene, E., Costantine, M. M., Buhimschi, C. S., Buhimschi, I., Saade, G. R., & Goharkhay, N. (2010). Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility. American journal of obstetrics and gynecology, 203(6), 596.e1-596.e5. https://doi.org/10.1016/j.ajog.2010.07.031

Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility. / Byers, Benjamin D.; Bytautiene, Egle; Costantine, Maged M. et al.
In: American journal of obstetrics and gynecology, Vol. 203, No. 6, 12.2010, p. 596.e1-596.e5.

Research output: Contribution to journal › Article › peer-review

Byers, BD, Bytautiene, E, Costantine, MM, Buhimschi, CS, Buhimschi, I, Saade, GR & Goharkhay, N 2010, 'Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility', American journal of obstetrics and gynecology, vol. 203, no. 6, pp. 596.e1-596.e5. https://doi.org/10.1016/j.ajog.2010.07.031

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abstract = "OBJECTIVE: To investigate the effect of intracervical hyaluronidase on the biomechanical properties of the cervix and on uterine contractility. STUDY DESIGN: Sprague-Dawley rats (n=33, term day 22) were injected with hyaluronidase (100 IU) or saline solution on day 8 of gestation (n=8-9/group). On day 21, labor was induced with mifepristone (8 mg/rat). Injection-to-delivery times were recorded. Biomechanical properties of the cervix were assessed using stretch-tension analysis. Myometrial contractility was investigated in response to hyaluronidase (0.2-200 IU/mL), oxytocin (10-10M to 10 -5M), and potassium chloride (60 mM). RESULTS: Delivery times were shorter in the hyaluronidase group (P= .03). Cervices of the treated animals showed higher measures of elasticity and plasticity (P= .02 for both). Myometrial sensitivity to hyaluronidase, oxytocin, or potassium chloride was not affected by the cervical application of hyaluronidase (P>.05 for all). CONCLUSION: Cervical hyaluronidase treatment shortens labor and alters the biomechanical properties of the cervix, independent of the myometrium.",

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AU - Buhimschi, Catalin S.

AU - Buhimschi, Irina

AU - Saade, George R.

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N2 - OBJECTIVE: To investigate the effect of intracervical hyaluronidase on the biomechanical properties of the cervix and on uterine contractility. STUDY DESIGN: Sprague-Dawley rats (n=33, term day 22) were injected with hyaluronidase (100 IU) or saline solution on day 8 of gestation (n=8-9/group). On day 21, labor was induced with mifepristone (8 mg/rat). Injection-to-delivery times were recorded. Biomechanical properties of the cervix were assessed using stretch-tension analysis. Myometrial contractility was investigated in response to hyaluronidase (0.2-200 IU/mL), oxytocin (10-10M to 10 -5M), and potassium chloride (60 mM). RESULTS: Delivery times were shorter in the hyaluronidase group (P= .03). Cervices of the treated animals showed higher measures of elasticity and plasticity (P= .02 for both). Myometrial sensitivity to hyaluronidase, oxytocin, or potassium chloride was not affected by the cervical application of hyaluronidase (P>.05 for all). CONCLUSION: Cervical hyaluronidase treatment shortens labor and alters the biomechanical properties of the cervix, independent of the myometrium.

AB - OBJECTIVE: To investigate the effect of intracervical hyaluronidase on the biomechanical properties of the cervix and on uterine contractility. STUDY DESIGN: Sprague-Dawley rats (n=33, term day 22) were injected with hyaluronidase (100 IU) or saline solution on day 8 of gestation (n=8-9/group). On day 21, labor was induced with mifepristone (8 mg/rat). Injection-to-delivery times were recorded. Biomechanical properties of the cervix were assessed using stretch-tension analysis. Myometrial contractility was investigated in response to hyaluronidase (0.2-200 IU/mL), oxytocin (10-10M to 10 -5M), and potassium chloride (60 mM). RESULTS: Delivery times were shorter in the hyaluronidase group (P= .03). Cervices of the treated animals showed higher measures of elasticity and plasticity (P= .02 for both). Myometrial sensitivity to hyaluronidase, oxytocin, or potassium chloride was not affected by the cervical application of hyaluronidase (P>.05 for all). CONCLUSION: Cervical hyaluronidase treatment shortens labor and alters the biomechanical properties of the cervix, independent of the myometrium.

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KW - Hyaluronidase

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KW - Myometrium

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Hyaluronidase modifies the biomechanical properties of the rat cervix and shortens the duration of labor independent of myometrial contractility

Abstract

Keywords

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