Human monoclonal antibody against colon cancer

Chong Zheng Yao; Jin Ishizuka; Richard J. Bold; Howard E. Sperling; Courtney M. Townsend; James C. Thompson

doi:10.3109/07357909609012141

Human monoclonal antibody against colon cancer

Chong Zheng Yao, Jin Ishizuka, Richard J. Bold, Howard E. Sperling, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The purpose of the study was to produce human monoclonal antibodies (hMcAb) against human colon cancer for use in radioimmunoimaging. Human-mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hybrid heteromyeloma cells with human lymphocytes from colon cancer tumor-draining lymph nodes. The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell fines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites. Biodistribution study demonstrated that specific uptake of ¹²⁵I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues. Radioimmunoimaging of human colon cancer xenografts exhibited distinct tumor visualization during the period of 72-96 hr after intraperitoneal injection of ¹²⁵I-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer.

Original language	English (US)
Pages (from-to)	211-217
Number of pages	7
Journal	Cancer Investigation
Volume	14
Issue number	3
DOIs	https://doi.org/10.3109/07357909609012141
State	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.3109/07357909609012141

Cite this

@article{bd8ad4ab5bdb499896c268f7e20cfc13,

title = "Human monoclonal antibody against colon cancer",

abstract = "The purpose of the study was to produce human monoclonal antibodies (hMcAb) against human colon cancer for use in radioimmunoimaging. Human-mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hybrid heteromyeloma cells with human lymphocytes from colon cancer tumor-draining lymph nodes. The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell fines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites. Biodistribution study demonstrated that specific uptake of 125I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues. Radioimmunoimaging of human colon cancer xenografts exhibited distinct tumor visualization during the period of 72-96 hr after intraperitoneal injection of 125I-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer.",

author = "Yao, {Chong Zheng} and Jin Ishizuka and Bold, {Richard J.} and Sperling, {Howard E.} and Townsend, {Courtney M.} and Thompson, {James C.}",

note = "Funding Information: This work was supported by funding from the National Institutes of Health (POI DK35608.51237 DK 15241). the American Cancer Society (CB-.571), and Walls Medical Research Foundation.",

year = "1996",

doi = "10.3109/07357909609012141",

language = "English (US)",

volume = "14",

pages = "211--217",

journal = "Cancer Investigation",

issn = "0735-7907",

publisher = "Informa Healthcare",

number = "3",

}

TY - JOUR

T1 - Human monoclonal antibody against colon cancer

AU - Yao, Chong Zheng

AU - Ishizuka, Jin

AU - Bold, Richard J.

AU - Sperling, Howard E.

AU - Townsend, Courtney M.

AU - Thompson, James C.

N1 - Funding Information: This work was supported by funding from the National Institutes of Health (POI DK35608.51237 DK 15241). the American Cancer Society (CB-.571), and Walls Medical Research Foundation.

PY - 1996

Y1 - 1996

N2 - The purpose of the study was to produce human monoclonal antibodies (hMcAb) against human colon cancer for use in radioimmunoimaging. Human-mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hybrid heteromyeloma cells with human lymphocytes from colon cancer tumor-draining lymph nodes. The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell fines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites. Biodistribution study demonstrated that specific uptake of 125I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues. Radioimmunoimaging of human colon cancer xenografts exhibited distinct tumor visualization during the period of 72-96 hr after intraperitoneal injection of 125I-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer.

AB - The purpose of the study was to produce human monoclonal antibodies (hMcAb) against human colon cancer for use in radioimmunoimaging. Human-mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hybrid heteromyeloma cells with human lymphocytes from colon cancer tumor-draining lymph nodes. The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell fines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites. Biodistribution study demonstrated that specific uptake of 125I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues. Radioimmunoimaging of human colon cancer xenografts exhibited distinct tumor visualization during the period of 72-96 hr after intraperitoneal injection of 125I-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer.

UR - http://www.scopus.com/inward/record.url?scp=0029784460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029784460&partnerID=8YFLogxK

U2 - 10.3109/07357909609012141

DO - 10.3109/07357909609012141

M3 - Article

C2 - 8630681

AN - SCOPUS:0029784460

SN - 0735-7907

VL - 14

SP - 211

EP - 217

JO - Cancer Investigation

JF - Cancer Investigation

IS - 3

ER -

Human monoclonal antibody against colon cancer

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this