TY - JOUR
T1 - Human intestinal cryptosporidiosis
T2 - Secretory diarrhea and enterotoxic activity in Caco-2 cells
AU - Guarino, Alfredo
AU - Canani, Roberte Berni
AU - Casola, Antonella
AU - Pozio, Edoardo
AU - Russo, Rosario
AU - Bruzzese, Eugenia
AU - Fontana, Massimo
AU - Rubino, Armido
N1 - Funding Information:
Received 26 July 1994; revised 21 November 1994. Informed consent was obtained from the patients from whom fecal samples were obtained. The study was approved by the Ethical Committee of the Department of Pediatrics. University Federico II of Naples. Grant support: Consiglio Nazionale delle Ricerche, target project "Biotechnology and Bioinstrumentation" (research program 9301081. P.F. 70); Ministero della Sanita, AIDS research project (1994) (programs 9205-30 and 920 W). Reprints or correspondence: Dr. Alfredo Guarino. Dept. of Pediatrics. University Federico II of Naples. Via S. Pansini, 580131 Naples. Italy.
PY - 1995/4
Y1 - 1995/4
N2 - A cell line model to detect enterotoxic effect was used to test fecal specimens of patients with enteric cryptosporidiosis. Fecal samples were obtained from 11 patients with Cryptosporidium diarrhea, and osmotic gap was determined. Caco-2 cell monolayers grown on filters were mounted in Ussing chambers, and electrical parameters were measured before and after the addition of fecal supernatant. A significant increase in short-circuit current was seen in 9 of 11 specimens. The enterotoxic effect was time- and dose-dependent, saturable, and Cl-- and Ca2+-dependent. Fecal osmotic gap was consistent with secretory diarrhea in the 9 enterotoxin-positive but not in the 2 enterotoxin-negative samples. In conclusion, a cell line model for studying the pathophysiology of enteric cryptosporidiosis was established. Enterotoxic activity was observed in most patients with enteric cryptosporidiosis and was strictly associated with secretory diarrhea.
AB - A cell line model to detect enterotoxic effect was used to test fecal specimens of patients with enteric cryptosporidiosis. Fecal samples were obtained from 11 patients with Cryptosporidium diarrhea, and osmotic gap was determined. Caco-2 cell monolayers grown on filters were mounted in Ussing chambers, and electrical parameters were measured before and after the addition of fecal supernatant. A significant increase in short-circuit current was seen in 9 of 11 specimens. The enterotoxic effect was time- and dose-dependent, saturable, and Cl-- and Ca2+-dependent. Fecal osmotic gap was consistent with secretory diarrhea in the 9 enterotoxin-positive but not in the 2 enterotoxin-negative samples. In conclusion, a cell line model for studying the pathophysiology of enteric cryptosporidiosis was established. Enterotoxic activity was observed in most patients with enteric cryptosporidiosis and was strictly associated with secretory diarrhea.
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U2 - 10.1093/infdis/171.4.976
DO - 10.1093/infdis/171.4.976
M3 - Article
C2 - 7706827
AN - SCOPUS:0028936051
SN - 0022-1899
VL - 171
SP - 976
EP - 983
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -