Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei

Gregory C. Whitlock; Roman A. Lukaszewski; Barbara M. Judy; Slobodan Paessler; Alfredo G. Torres; D. Mark Estes

doi:10.1186/1471-2172-9-55

Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei

Gregory C. Whitlock, Roman A. Lukaszewski, Barbara M. Judy, Slobodan Paessler, Alfredo G. Torres, D. Mark Estes

Pathology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Background: We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection. Results: While protection with heat-killed bacilli did not result in sterilizing immunity, limited protection was afforded against an otherwise lethal infection and provided insight into potential host protective mechanisms. Our results demonstrated that mice depleted of either B cells, TNF-α or IFN-γ exhibited decreased survival rates, indicating a role for these effectors in obtaining partial protection from a lethal challenge by the intraperitoneal route. Additionally, complement depletion had no effect on immunoglobulin production when compared to non-complement depleted controls infected intranasally. Conclusion: The data provide a basis for future studies of protection via vaccination using either subunit or whole-organism vaccine preparations from lethal infection in the experimental BALB/c mouse model. The results of this study demonstrate participation of B220⁺ cells and pro-inflammatory cytokines IFN-γ and TNF-α in protection following HK vaccination.

Original language	English (US)
Article number	55
Journal	BMC Immunology
Volume	9
DOIs	https://doi.org/10.1186/1471-2172-9-55
State	Published - Sep 29 2008

ASJC Scopus subject areas

Immunology

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title = "Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei",

abstract = "Background: We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection. Results: While protection with heat-killed bacilli did not result in sterilizing immunity, limited protection was afforded against an otherwise lethal infection and provided insight into potential host protective mechanisms. Our results demonstrated that mice depleted of either B cells, TNF-α or IFN-γ exhibited decreased survival rates, indicating a role for these effectors in obtaining partial protection from a lethal challenge by the intraperitoneal route. Additionally, complement depletion had no effect on immunoglobulin production when compared to non-complement depleted controls infected intranasally. Conclusion: The data provide a basis for future studies of protection via vaccination using either subunit or whole-organism vaccine preparations from lethal infection in the experimental BALB/c mouse model. The results of this study demonstrate participation of B220+ cells and pro-inflammatory cytokines IFN-γ and TNF-α in protection following HK vaccination.",

author = "Whitlock, {Gregory C.} and Lukaszewski, {Roman A.} and Judy, {Barbara M.} and Slobodan Paessler and Torres, {Alfredo G.} and Estes, {D. Mark}",

note = "Funding Information: The authors thank Drs. Mitali Sarkar-Tyson and Richard W. Titball for helpful discussions and Lifang Zhang for statistical analysis. This work was supported by NIH grant U54 AI057156 (D.M.E.) and a fellowship award to G.C.W. from the Sealy Center for Vaccine Development.",

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doi = "10.1186/1471-2172-9-55",

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AU - Whitlock, Gregory C.

AU - Lukaszewski, Roman A.

AU - Judy, Barbara M.

AU - Paessler, Slobodan

AU - Torres, Alfredo G.

AU - Estes, D. Mark

N1 - Funding Information: The authors thank Drs. Mitali Sarkar-Tyson and Richard W. Titball for helpful discussions and Lifang Zhang for statistical analysis. This work was supported by NIH grant U54 AI057156 (D.M.E.) and a fellowship award to G.C.W. from the Sealy Center for Vaccine Development.

PY - 2008/9/29

Y1 - 2008/9/29

N2 - Background: We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection. Results: While protection with heat-killed bacilli did not result in sterilizing immunity, limited protection was afforded against an otherwise lethal infection and provided insight into potential host protective mechanisms. Our results demonstrated that mice depleted of either B cells, TNF-α or IFN-γ exhibited decreased survival rates, indicating a role for these effectors in obtaining partial protection from a lethal challenge by the intraperitoneal route. Additionally, complement depletion had no effect on immunoglobulin production when compared to non-complement depleted controls infected intranasally. Conclusion: The data provide a basis for future studies of protection via vaccination using either subunit or whole-organism vaccine preparations from lethal infection in the experimental BALB/c mouse model. The results of this study demonstrate participation of B220+ cells and pro-inflammatory cytokines IFN-γ and TNF-α in protection following HK vaccination.

AB - Background: We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection. Results: While protection with heat-killed bacilli did not result in sterilizing immunity, limited protection was afforded against an otherwise lethal infection and provided insight into potential host protective mechanisms. Our results demonstrated that mice depleted of either B cells, TNF-α or IFN-γ exhibited decreased survival rates, indicating a role for these effectors in obtaining partial protection from a lethal challenge by the intraperitoneal route. Additionally, complement depletion had no effect on immunoglobulin production when compared to non-complement depleted controls infected intranasally. Conclusion: The data provide a basis for future studies of protection via vaccination using either subunit or whole-organism vaccine preparations from lethal infection in the experimental BALB/c mouse model. The results of this study demonstrate participation of B220+ cells and pro-inflammatory cytokines IFN-γ and TNF-α in protection following HK vaccination.

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Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei

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