HIV infection of primary CD4+ TH2 cells, defined by expression of the chemoattractant receptor-homologous (CRTH2), induces a Th0 phenotype

Bouchaib Bahbouhi, Alan Landay, Allan Tenorio, Lena Al-Harthi

Research output: Contribution to journalArticlepeer-review

Abstract

The association between HIV, cytokine profile, and disease progression is controversial. In this study, we evaluated whether HIV infection of a primary T helper-like type 2 cytokine (Th2) cell subset augments their cytokine profile. We utilized the CRTH2 (chemoattractant receptor-homologous) marker to identify CD4+Th2 cells. Approximately 2-4% of CD4+ T cells are CRTH2+. CRTH2+ expression is confirmed to delineate a Th2 subset as indicated by robust inducible IL-4 response. CD4+CRTH2 + T cells were also more inherently activated than their CRTH2-negative counterpart as indicated by a higher percent expression of CD69, CD45RO, CD95, CD25, and HLA-DR. CD4+CRTH2+ T cells were not terminally differentiated as indicated by expression of CD27 and CD28. In vitro HIV infection of primary human CD4+CRTH2+ T cells, independent of chemokine coreceptor usage, potently upregulated IFN-γ production while still maintaining robust IL-4 expression. This Th0 (IFNγ+IL-4+) phenotype was upregulated in CD4 +CRTH2+ T cells post-HIV infection by 18-fold, demonstrating a shift to a Th0 phenotype. Ex vivo studies also demonstrated that HIV+ patients exhibited a decline in CD4+CRTH2 + cells and a shift of this population toward cells that express both IFN-γ and IL-4. Collectively, these data indicate that HIV replication in Th2 cells induces a Th0 phenotype. This phenomenon may be a deliberate viral escape mechanism to prevent the skewing of the immune response toward Th1 or Th2.

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume23
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'HIV infection of primary CD4+ TH2 cells, defined by expression of the chemoattractant receptor-homologous (CRTH2), induces a Th0 phenotype'. Together they form a unique fingerprint.

Cite this