HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women’s Interagency HIV study

Juan Lin, Erik Ehinger, David B. Hanna, Qibin Qi, Tao Wang, Yanal Ghosheh, Karin Mueller, Kathryn Anastos, Jason M. Lazar, Wendy J. Mack, Phyllis C. Tien, Joan W. Berman, Mardge H. Cohen, Igho Ofotokun, Stephen Gange, Chenglong Liu, Sonya L. Heath, Russell P. Tracy, Howard N. Hodis, Alan L. LandayKlaus Ley, Robert C. Kaplan

Research output: Contribution to journalArticlepeer-review


Persistent inflammation contributes to the development of cardiovascular disease (CVD) as an HIV-associated comorbidity. Innate immune cells such as monocytes are major drivers of inflammation in men and women with HIV. The study objectives are to examine the contribution of circulating non-classical monocytes (NCM, CD14dimCD16+) and intermediate monocytes (IM, CD14+CD16+) to the host response to long-term HIV infection and HIV-associated CVD. Women with and without chronic HIV infection (H) were studied. Subclinical CVD (C) was detected as plaques imaged by B-mode carotid artery ultrasound. The study included H-C-, H+C-, H-C+, and H+C+ participants (23 of each, matched on race/ethnicity, age and smoking status), selected from among enrollees in the Women’s Interagency HIV Study. We assessed transcriptomic features associated with HIV or CVD alone or comorbid HIV/CVD comparing to healthy (H-C-) participants in IM and NCM isolated from peripheral blood mononuclear cells. IM gene expression was little affected by HIV alone or CVD alone. In IM, coexisting HIV and CVD produced a measurable gene transcription signature, which was abolished by lipid-lowering treatment. In NCM, versus non-HIV controls, women with HIV had altered gene expression, irrespective of whether or not they had comorbid CVD. The largest set of differentially expressed genes was found in NCM among women with both HIV and CVD. Genes upregulated in association with HIV included several potential targets of drug therapies, including LAG3 (CD223). In conclusion, circulating monocytes from patients with well controlled HIV infection demonstrate an extensive gene expression signature which may be consistent with the ability of these cells to serve as potential viral reservoirs. Gene transcriptional changes in HIV patients were further magnified in the presence of subclinical CVD.

Original languageEnglish (US)
Article numbere0285926
JournalPloS one
Issue number5 May
StatePublished - May 2023
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women’s Interagency HIV study'. Together they form a unique fingerprint.

Cite this