Histamine releasing activity (HRA) produced by leukocytes co-cultured with tumor cells

Marlyn P. Langford, Micheal A. Lett-Brown, G. John Stanton, J. Andrew Grant

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Human stomach adenocarcinoma (AGS), astrocytoma (AST) and mylogenous leukemia (K562) cells were co-cultured with human peripheral blood leukocytes (PBL) through four days. Histamine releasing activity (HRA, lymphokines that stimulate degranulation of basophilic leukocytes with the release of histamine) and interferons alpha and gamma (IFNα and IFNγ were detected in the culture fluids. Maximal levels of HRA were detected by 24 hr in AST and K562-leukocyte co-culture fluids. Notably, only low levels of HRA was detected in AGS-leukocyte cultures. HRA was separated into two molecular weight species (60,000 and 25,000) by column chromatography. The IFN activity was shown to be a mixture of IFNα and IFNγ (IFNαgt;IFNγ IFN did not cause histamine release from human PEL or affect HRA. Our results indicate that PBL from normal individuals produce HRA in response to tumor antigen and suggest that the basophilic leukocyte response to certain cancers may be related to HRA production.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalImmunological Investigations
Issue number2
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Immunology


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