High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion

Zee Yong Park; Rovshan Sadygov; John R. Yates

High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion

Zee Yong Park, Rovshan Sadygov, John R. Yates

Research output: Contribution to conference › Paper › peer-review

Abstract

The use of computer algorithm for analyzing the cleavage pattern by mass spectrometry to map disulfide bonds was discussed. This labeling method had shown to be effective especially in mapping complicated disulfide bond patterns of adjacent cysteins. In the latest disulfide bond mapping method minimum sample preparation and computer based data analysis were used. Results show that three disulfide bonds of lysozyme and ten disulfide bonds of bovine serum albumin were easily mapped by the method without human intervention.

Original language	English (US)
Pages	43-44
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

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High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. / Park, Zee Yong; Sadygov, Rovshan; Yates, John R.
2002. 43-44 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "The use of computer algorithm for analyzing the cleavage pattern by mass spectrometry to map disulfide bonds was discussed. This labeling method had shown to be effective especially in mapping complicated disulfide bond patterns of adjacent cysteins. In the latest disulfide bond mapping method minimum sample preparation and computer based data analysis were used. Results show that three disulfide bonds of lysozyme and ten disulfide bonds of bovine serum albumin were easily mapped by the method without human intervention.",

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AU - Park, Zee Yong

AU - Sadygov, Rovshan

AU - Yates, John R.

PY - 2002

Y1 - 2002

N2 - The use of computer algorithm for analyzing the cleavage pattern by mass spectrometry to map disulfide bonds was discussed. This labeling method had shown to be effective especially in mapping complicated disulfide bond patterns of adjacent cysteins. In the latest disulfide bond mapping method minimum sample preparation and computer based data analysis were used. Results show that three disulfide bonds of lysozyme and ten disulfide bonds of bovine serum albumin were easily mapped by the method without human intervention.

AB - The use of computer algorithm for analyzing the cleavage pattern by mass spectrometry to map disulfide bonds was discussed. This labeling method had shown to be effective especially in mapping complicated disulfide bond patterns of adjacent cysteins. In the latest disulfide bond mapping method minimum sample preparation and computer based data analysis were used. Results show that three disulfide bonds of lysozyme and ten disulfide bonds of bovine serum albumin were easily mapped by the method without human intervention.

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M3 - Paper

AN - SCOPUS:2442699331

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T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion

Abstract

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ASJC Scopus subject areas

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