TY - JOUR
T1 - High-performance liquid chromatography method for the determination of protein-glutathione mixed disulfide
AU - Kumari, Kshama
AU - Khanna, Peeyush
AU - Ansari, Naseem H.
AU - Srivastava, Satish K.
PY - 1994/8/1
Y1 - 1994/8/1
N2 - We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.
AB - We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.
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U2 - 10.1006/abio.1994.1352
DO - 10.1006/abio.1994.1352
M3 - Article
C2 - 7978281
AN - SCOPUS:0028128232
SN - 0003-2697
VL - 220
SP - 374
EP - 376
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -