Abstract
Insulin-like growth factor (IGF)II is a mitogenic polypeptide which circulates in association with a binding proteins(s). Immunoblotting studies were performed in human serum and indicate that:(1)a ∼200 kDa covalently-linked IGF-II/binding protein complex in antigenically related to the 30 kDa binding protein, (2)IGF-II prohormone is associated with this complex, and (3)a major portion of the IGF-II prohormone immunoreactivity in human serum is present in fractions which would not be detected by standard radioimmunoassay methods. Our data provide insight regarding the inter-relationships of IGF-II and its binding protein, and direct evidence for the presence of IGF-II prohormone in human serum.
Original language | English (US) |
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Pages (from-to) | 1131-1137 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 152 |
Issue number | 3 |
DOIs | |
State | Published - May 16 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology