Heat induced oxidative stress and aberrations in liver function leading to hepatic injury in rats

Avinash Gupta; Nishant Ranjan Chauhan; Ajeet Singh; Daipayan Chowdhury; Ramesh Chand Meena; Amitabha Chakrabarti; Lilly Ganju; Bhuvnesh Kumar; Shashi Bala Singh

doi:10.14429/dlsj.4.13174

Heat induced oxidative stress and aberrations in liver function leading to hepatic injury in rats

Avinash Gupta, Nishant Ranjan Chauhan, Ajeet Singh, Daipayan Chowdhury, Ramesh Chand Meena, Amitabha Chakrabarti, Lilly Ganju, Bhuvnesh Kumar, Shashi Bala Singh

Research output: Contribution to journal › Article › peer-review

Abstract

Exposure to heat stress (HS) elicits systemic and cellular response in experimental animals and humans. The current study was undertaken to determine the effect of HS on liver microstructure and function in rats. A heat simulation chamber with ambient temperature (T_a) 45 ± 0.5 °C and relative humidity (RH) 30 ± 5 per cent was used to expose animals to HS. Rats were categorised as moderately heat stressed (MHS, T_c = 40 °C) and severely heat stressed (SHS, T_c = 42 °C) groups. We observed that with rise in core temperature (T_c) alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were increased but glucose level was decreased in both plasma and liver tissue. Significantly elevated levels of reactive oxygen species (ROS) and nitric oxide (NO) were detected in liver of MHS and SHS animals. Additionally, glutathione disulfide and glutathione (GSSG and GSH) ratio was found to be increased with rise in T_c which suggested saturation in antioxidant capacity of tissue. Furthermore, levels of heat shock proteins (HSPs) and caspases were upregulated upon HS. Results of histological examination indicated extensive loss of cells in liver parenchyma leading to disorganisation of lobular structure. Thus, biochemical and histological studies in experimental animals demonstrates that HS may severely altered structural and biochemical functions of liver.

Original language	English (US)
Pages (from-to)	21-32
Number of pages	12
Journal	Defence Life Science Journal
Volume	4
Issue number	1
DOIs	https://doi.org/10.14429/dlsj.4.13174
State	Published - Jan 2019
Externally published	Yes

Keywords

Heat shock proteins
Heat stress
Liver
Liver function test
Oxidative stress

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Pharmacology, Toxicology and Pharmaceutics

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10.14429/dlsj.4.13174

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title = "Heat induced oxidative stress and aberrations in liver function leading to hepatic injury in rats",

abstract = "Exposure to heat stress (HS) elicits systemic and cellular response in experimental animals and humans. The current study was undertaken to determine the effect of HS on liver microstructure and function in rats. A heat simulation chamber with ambient temperature (Ta) 45 ± 0.5 °C and relative humidity (RH) 30 ± 5 per cent was used to expose animals to HS. Rats were categorised as moderately heat stressed (MHS, Tc = 40 °C) and severely heat stressed (SHS, Tc = 42 °C) groups. We observed that with rise in core temperature (Tc) alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were increased but glucose level was decreased in both plasma and liver tissue. Significantly elevated levels of reactive oxygen species (ROS) and nitric oxide (NO) were detected in liver of MHS and SHS animals. Additionally, glutathione disulfide and glutathione (GSSG and GSH) ratio was found to be increased with rise in Tc which suggested saturation in antioxidant capacity of tissue. Furthermore, levels of heat shock proteins (HSPs) and caspases were upregulated upon HS. Results of histological examination indicated extensive loss of cells in liver parenchyma leading to disorganisation of lobular structure. Thus, biochemical and histological studies in experimental animals demonstrates that HS may severely altered structural and biochemical functions of liver.",

keywords = "Heat shock proteins, Heat stress, Liver, Liver function test, Oxidative stress",

author = "Avinash Gupta and Chauhan, {Nishant Ranjan} and Ajeet Singh and Daipayan Chowdhury and Meena, {Ramesh Chand} and Amitabha Chakrabarti and Lilly Ganju and Bhuvnesh Kumar and Singh, {Shashi Bala}",

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T1 - Heat induced oxidative stress and aberrations in liver function leading to hepatic injury in rats

AU - Gupta, Avinash

AU - Chauhan, Nishant Ranjan

AU - Singh, Ajeet

AU - Chowdhury, Daipayan

AU - Meena, Ramesh Chand

AU - Chakrabarti, Amitabha

AU - Ganju, Lilly

AU - Kumar, Bhuvnesh

AU - Singh, Shashi Bala

PY - 2019/1

Y1 - 2019/1

N2 - Exposure to heat stress (HS) elicits systemic and cellular response in experimental animals and humans. The current study was undertaken to determine the effect of HS on liver microstructure and function in rats. A heat simulation chamber with ambient temperature (Ta) 45 ± 0.5 °C and relative humidity (RH) 30 ± 5 per cent was used to expose animals to HS. Rats were categorised as moderately heat stressed (MHS, Tc = 40 °C) and severely heat stressed (SHS, Tc = 42 °C) groups. We observed that with rise in core temperature (Tc) alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were increased but glucose level was decreased in both plasma and liver tissue. Significantly elevated levels of reactive oxygen species (ROS) and nitric oxide (NO) were detected in liver of MHS and SHS animals. Additionally, glutathione disulfide and glutathione (GSSG and GSH) ratio was found to be increased with rise in Tc which suggested saturation in antioxidant capacity of tissue. Furthermore, levels of heat shock proteins (HSPs) and caspases were upregulated upon HS. Results of histological examination indicated extensive loss of cells in liver parenchyma leading to disorganisation of lobular structure. Thus, biochemical and histological studies in experimental animals demonstrates that HS may severely altered structural and biochemical functions of liver.

AB - Exposure to heat stress (HS) elicits systemic and cellular response in experimental animals and humans. The current study was undertaken to determine the effect of HS on liver microstructure and function in rats. A heat simulation chamber with ambient temperature (Ta) 45 ± 0.5 °C and relative humidity (RH) 30 ± 5 per cent was used to expose animals to HS. Rats were categorised as moderately heat stressed (MHS, Tc = 40 °C) and severely heat stressed (SHS, Tc = 42 °C) groups. We observed that with rise in core temperature (Tc) alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were increased but glucose level was decreased in both plasma and liver tissue. Significantly elevated levels of reactive oxygen species (ROS) and nitric oxide (NO) were detected in liver of MHS and SHS animals. Additionally, glutathione disulfide and glutathione (GSSG and GSH) ratio was found to be increased with rise in Tc which suggested saturation in antioxidant capacity of tissue. Furthermore, levels of heat shock proteins (HSPs) and caspases were upregulated upon HS. Results of histological examination indicated extensive loss of cells in liver parenchyma leading to disorganisation of lobular structure. Thus, biochemical and histological studies in experimental animals demonstrates that HS may severely altered structural and biochemical functions of liver.

KW - Heat shock proteins

KW - Heat stress

KW - Liver

KW - Liver function test

KW - Oxidative stress

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Heat induced oxidative stress and aberrations in liver function leading to hepatic injury in rats

Abstract

Keywords

ASJC Scopus subject areas

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