Grafting of leg ulcers with undifferentiated keratinocytes

Miriam M. Brysk; Sharon S. Raimer; Rafael Pupo; Trace Bell; Srinivasan Rajaraman

doi:10.1016/0190-9622(91)70188-8

Grafting of leg ulcers with undifferentiated keratinocytes

Miriam M. Brysk, Sharon S. Raimer, Rafael Pupo, Trace Bell, Srinivasan Rajaraman

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A procedure for transplanting cultured human keratinocytes has been developed and used successfully to treat patients with persistent leg ulcers. Shave excisions of donor skin of 1 cm2 or less were expanded in culture in l week to cover wounds as large as 90 cm². The uniqueness of this system is the use of a biochemically modified surgical dressing that permits the transplantation of undifferentiated cells. Several hours after inoculation, trypsinized single cells become attached to the dressing and are ready for grafting. Seven of eight persistent stasis ulcers completely reepithelialized after application of the modified surgical dressing with undifferentiated keratinocytes.

Original language	English (US)
Pages (from-to)	238-244
Number of pages	7
Journal	Journal of the American Academy of Dermatology
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/0190-9622(91)70188-8
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Dermatology

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10.1016/0190-9622(91)70188-8

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title = "Grafting of leg ulcers with undifferentiated keratinocytes",

abstract = "A procedure for transplanting cultured human keratinocytes has been developed and used successfully to treat patients with persistent leg ulcers. Shave excisions of donor skin of 1 cm2 or less were expanded in culture in l week to cover wounds as large as 90 cm2. The uniqueness of this system is the use of a biochemically modified surgical dressing that permits the transplantation of undifferentiated cells. Several hours after inoculation, trypsinized single cells become attached to the dressing and are ready for grafting. Seven of eight persistent stasis ulcers completely reepithelialized after application of the modified surgical dressing with undifferentiated keratinocytes.",

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AU - Raimer, Sharon S.

AU - Pupo, Rafael

AU - Bell, Trace

AU - Rajaraman, Srinivasan

PY - 1991

Y1 - 1991

N2 - A procedure for transplanting cultured human keratinocytes has been developed and used successfully to treat patients with persistent leg ulcers. Shave excisions of donor skin of 1 cm2 or less were expanded in culture in l week to cover wounds as large as 90 cm2. The uniqueness of this system is the use of a biochemically modified surgical dressing that permits the transplantation of undifferentiated cells. Several hours after inoculation, trypsinized single cells become attached to the dressing and are ready for grafting. Seven of eight persistent stasis ulcers completely reepithelialized after application of the modified surgical dressing with undifferentiated keratinocytes.

AB - A procedure for transplanting cultured human keratinocytes has been developed and used successfully to treat patients with persistent leg ulcers. Shave excisions of donor skin of 1 cm2 or less were expanded in culture in l week to cover wounds as large as 90 cm2. The uniqueness of this system is the use of a biochemically modified surgical dressing that permits the transplantation of undifferentiated cells. Several hours after inoculation, trypsinized single cells become attached to the dressing and are ready for grafting. Seven of eight persistent stasis ulcers completely reepithelialized after application of the modified surgical dressing with undifferentiated keratinocytes.

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Grafting of leg ulcers with undifferentiated keratinocytes

Abstract

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