Glycine potentiates N-methyl-d-aspartate-induced [3H]TCP binding to rat cortical membranes

Lawrence D. Snell; Robert S. Morter; Kenneth M. Johnson

doi:10.1016/0304-3940(87)90106-6

Glycine potentiates N-methyl-d-aspartate-induced [³H]TCP binding to rat cortical membranes

Lawrence D. Snell, Robert S. Morter, Kenneth M. Johnson

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

In extensively washed preparations of rat cortical membranes, N-methyl-d-aspartate (NMDA) increases the specific binding of [³H]TCP by over 4-fold in a concentration dependent manner (EC₅₀ = 3.1 μM). Glycine (1 μM) potentiates the maximal effect of NMDA by a factor of 1.7. The effect of glycine is concentration dependent (EC₅₀ = 380 nM) and strychnine insensitive. These data are discussed with reference to the recently reported effects of glycine on the NMDA operated cation channel and the relationship between the PCP and NMDA receptors.

Original language	English (US)
Pages (from-to)	313-317
Number of pages	5
Journal	Neuroscience Letters
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(87)90106-6
State	Published - Dec 29 1987
Externally published	Yes

Keywords

1-[1-(2-Thienyl)cyclohexyl]piperidine hydrochloride (TCP)
Glycine
N-Methyl-d-aspartate
PCP/σ-receptor
Phencyclidine (PCP)
Strychnine

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(87)90106-6

Cite this

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title = "Glycine potentiates N-methyl-d-aspartate-induced [3H]TCP binding to rat cortical membranes",

abstract = "In extensively washed preparations of rat cortical membranes, N-methyl-d-aspartate (NMDA) increases the specific binding of [3H]TCP by over 4-fold in a concentration dependent manner (EC50 = 3.1 μM). Glycine (1 μM) potentiates the maximal effect of NMDA by a factor of 1.7. The effect of glycine is concentration dependent (EC50 = 380 nM) and strychnine insensitive. These data are discussed with reference to the recently reported effects of glycine on the NMDA operated cation channel and the relationship between the PCP and NMDA receptors.",

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T1 - Glycine potentiates N-methyl-d-aspartate-induced [3H]TCP binding to rat cortical membranes

AU - Snell, Lawrence D.

AU - Morter, Robert S.

AU - Johnson, Kenneth M.

PY - 1987/12/29

Y1 - 1987/12/29

N2 - In extensively washed preparations of rat cortical membranes, N-methyl-d-aspartate (NMDA) increases the specific binding of [3H]TCP by over 4-fold in a concentration dependent manner (EC50 = 3.1 μM). Glycine (1 μM) potentiates the maximal effect of NMDA by a factor of 1.7. The effect of glycine is concentration dependent (EC50 = 380 nM) and strychnine insensitive. These data are discussed with reference to the recently reported effects of glycine on the NMDA operated cation channel and the relationship between the PCP and NMDA receptors.

AB - In extensively washed preparations of rat cortical membranes, N-methyl-d-aspartate (NMDA) increases the specific binding of [3H]TCP by over 4-fold in a concentration dependent manner (EC50 = 3.1 μM). Glycine (1 μM) potentiates the maximal effect of NMDA by a factor of 1.7. The effect of glycine is concentration dependent (EC50 = 380 nM) and strychnine insensitive. These data are discussed with reference to the recently reported effects of glycine on the NMDA operated cation channel and the relationship between the PCP and NMDA receptors.

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KW - Glycine

KW - N-Methyl-d-aspartate

KW - PCP/σ-receptor

KW - Phencyclidine (PCP)

KW - Strychnine

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