Genotype 1 JEV was isolated again from Liaoning Province, China, 2007

Wei shan Meng, Ming hua Li, Huan yu Wang, Ji bo Zhang, Y. Gao, Jun Ding, Shi hong Fu, You gang Zhai, Zhe Chen, Jun qiao Gou, Yu jun Zhao, Guo dong Liang

Research output: Contribution to journalArticlepeer-review


OBJECTIVE: To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene. METHODS: Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods. RESULTS: Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002. CONCLUSION: Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
JournalZhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
Issue number2
StatePublished - Apr 2008
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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