Genome sequence diversity and clues to the evolution of variola (smallpox) virus

Joseph J. Esposito; Scott A. Sammons; A. Michael Frace; John D. Osborne; Melissa Glsen-Rasmussen; Ming Zhang; Dhwani Govil; Inger K. Damon; Richard Kline; Miriam Laker; Yu Li; Geoffrey L. Smith; Hermann Meyer; James W. LeDuc; Robert M. Wohlhueter

doi:10.1126/science.1125134

Genome sequence diversity and clues to the evolution of variola (smallpox) virus

Joseph J. Esposito, Scott A. Sammons, A. Michael Frace, John D. Osborne, Melissa Glsen-Rasmussen, Ming Zhang, Dhwani Govil, Inger K. Damon, Richard Kline, Miriam Laker, Yu Li, Geoffrey L. Smith, Hermann Meyer, James W. LeDuc, Robert M. Wohlhueter

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

Original language	English (US)
Pages (from-to)	807-812
Number of pages	6
Journal	Science
Volume	313
Issue number	5788
DOIs	https://doi.org/10.1126/science.1125134
State	Published - Aug 11 2006
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1125134

Cite this

@article{812dcc585eb5420bbb5c5359d7045d5f,

title = "Genome sequence diversity and clues to the evolution of variola (smallpox) virus",

abstract = "Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.",

author = "Esposito, {Joseph J.} and Sammons, {Scott A.} and Frace, {A. Michael} and Osborne, {John D.} and Melissa Glsen-Rasmussen and Ming Zhang and Dhwani Govil and Damon, {Inger K.} and Richard Kline and Miriam Laker and Yu Li and Smith, {Geoffrey L.} and Hermann Meyer and LeDuc, {James W.} and Wohlhueter, {Robert M.}",

year = "2006",

month = aug,

day = "11",

doi = "10.1126/science.1125134",

language = "English (US)",

volume = "313",

pages = "807--812",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5788",

}

TY - JOUR

T1 - Genome sequence diversity and clues to the evolution of variola (smallpox) virus

AU - Esposito, Joseph J.

AU - Sammons, Scott A.

AU - Frace, A. Michael

AU - Osborne, John D.

AU - Glsen-Rasmussen, Melissa

AU - Zhang, Ming

AU - Govil, Dhwani

AU - Damon, Inger K.

AU - Kline, Richard

AU - Laker, Miriam

AU - Li, Yu

AU - Smith, Geoffrey L.

AU - Meyer, Hermann

AU - LeDuc, James W.

AU - Wohlhueter, Robert M.

PY - 2006/8/11

Y1 - 2006/8/11

N2 - Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

AB - Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

UR - http://www.scopus.com/inward/record.url?scp=33747106195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747106195&partnerID=8YFLogxK

U2 - 10.1126/science.1125134

DO - 10.1126/science.1125134

M3 - Article

C2 - 16873609

AN - SCOPUS:33747106195

SN - 0036-8075

VL - 313

SP - 807

EP - 812

JO - Science

JF - Science

IS - 5788

ER -

Genome sequence diversity and clues to the evolution of variola (smallpox) virus

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this