TY - JOUR
T1 - Genetic variation in related cytolytic toxins produced by different species of Aeromonas
AU - Chopra, Ashok K.
AU - Houston, Clifford W.
AU - Kurosky, Alexander
N1 - Funding Information:
This investigation was supported by a Public Health Service grant DHHS 2RO1 AI21075 from the National Institute of Health to C.W.H. We thank Dr. Gui-Fang Jin for her help in raising polyclonal antobodies to the synthetic peptide. Critical comments of Dr. J.W. Peterson, in improving the manuscript is duly acknowledged. We thank Drs. J.T. Buckley, G. Stelma, and T. Chak-raborty for providing the various isolates and antisera utilized herein.
PY - 1991/3/1
Y1 - 1991/3/1
N2 - Comparative analyses were performed at the immunological, biological, and genetic level to establish the degree of relatedness of a group of four cytotoxic enterotoxins and aerolysins produced by different isolates of Aeromonas: SSU [16], Ah1 [13], Ah2 [18], and Ah65 [21]. Results obtained from Western blot analysis, neutralization studies, and Southern blot analysis indicated that although the members of this group of toxins displayed structural similarities they also differed from each other at the immunological, biological and genetic level.
AB - Comparative analyses were performed at the immunological, biological, and genetic level to establish the degree of relatedness of a group of four cytotoxic enterotoxins and aerolysins produced by different isolates of Aeromonas: SSU [16], Ah1 [13], Ah2 [18], and Ah65 [21]. Results obtained from Western blot analysis, neutralization studies, and Southern blot analysis indicated that although the members of this group of toxins displayed structural similarities they also differed from each other at the immunological, biological and genetic level.
KW - Aerolysin
KW - Cytotoxic enterotoxin
KW - Immunocrossreactivity
KW - Neutralization
KW - Southern blot hybridization
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U2 - 10.1111/j.1574-6968.1991.tb04448.x
DO - 10.1111/j.1574-6968.1991.tb04448.x
M3 - Article
C2 - 2040431
AN - SCOPUS:0026129021
SN - 0378-1097
VL - 78
SP - 231
EP - 238
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2-3
ER -