Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

S. L. Rossi; F. Nasar; J. Cardosa; S. V. Mayer; R. B. Tesh; K. A. Hanley; S. C. Weaver; Nikos Vasilakis

doi:10.1016/j.virol.2011.11.018

Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

S. L. Rossi, F. Nasar, J. Cardosa, S. V. Mayer, R. B. Tesh, K. A. Hanley, S. C. Weaver, Nikos Vasilakis

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.

Original language	English (US)
Pages (from-to)	58-67
Number of pages	10
Journal	Virology
Volume	423
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.11.018
State	Published - Feb 5 2012

Keywords

Dengue virus (DENV)
Human DENV
Phylogenetic and phenotypic analyses
Sylvatic DENV

ASJC Scopus subject areas

Virology

Access to Document

10.1016/j.virol.2011.11.018

Cite this

@article{83cd2d84df944f0e8262e61b76cc9135,

title = "Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains",

abstract = "Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.",

keywords = "Dengue virus (DENV), Human DENV, Phylogenetic and phenotypic analyses, Sylvatic DENV",

author = "Rossi, {S. L.} and F. Nasar and J. Cardosa and Mayer, {S. V.} and Tesh, {R. B.} and Hanley, {K. A.} and Weaver, {S. C.} and Nikos Vasilakis",

note = "Funding Information: We thank Naomi Forester and Thomas Monath for helpful comments on the manuscript, and The Japanese Health Sciences Foundation, Osaka, for providing the human hepatoma Huh-7 cells (clone JTC-39). SR is supported by 2T32AI007536-11A1 postdoctoral training grant; SW is supported for dengue research by NIH grant RO1 AI069145 ; KH is supported by NIH grants RO1 AI069145 and P20RR016480 and a Woods Hole Marine Biological Laboratory Karush Library Fellowship ; RT is supported by NIH contract HHSN272201000040I/HHSN27200004/D04 ; NV was supported by start-up funds provided by the Department of Pathology, UTMB . This work was presented in part at the 'Emerging and Re-emerging Vector-Borne and Zoonotic Viral Infectious Diseases in Southeast Asia', meeting in Hanoi, Vietnam, September 2010 and at the 58th Annual Meeting of the American Society for Tropical Medicine and Hygiene, Atlanta, November 2010.",

year = "2012",

month = feb,

day = "5",

doi = "10.1016/j.virol.2011.11.018",

language = "English (US)",

volume = "423",

pages = "58--67",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

AU - Rossi, S. L.

AU - Nasar, F.

AU - Cardosa, J.

AU - Mayer, S. V.

AU - Tesh, R. B.

AU - Hanley, K. A.

AU - Weaver, S. C.

AU - Vasilakis, Nikos

N1 - Funding Information: We thank Naomi Forester and Thomas Monath for helpful comments on the manuscript, and The Japanese Health Sciences Foundation, Osaka, for providing the human hepatoma Huh-7 cells (clone JTC-39). SR is supported by 2T32AI007536-11A1 postdoctoral training grant; SW is supported for dengue research by NIH grant RO1 AI069145 ; KH is supported by NIH grants RO1 AI069145 and P20RR016480 and a Woods Hole Marine Biological Laboratory Karush Library Fellowship ; RT is supported by NIH contract HHSN272201000040I/HHSN27200004/D04 ; NV was supported by start-up funds provided by the Department of Pathology, UTMB . This work was presented in part at the 'Emerging and Re-emerging Vector-Borne and Zoonotic Viral Infectious Diseases in Southeast Asia', meeting in Hanoi, Vietnam, September 2010 and at the 58th Annual Meeting of the American Society for Tropical Medicine and Hygiene, Atlanta, November 2010.

PY - 2012/2/5

Y1 - 2012/2/5

N2 - Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.

AB - Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.

KW - Dengue virus (DENV)

KW - Human DENV

KW - Phylogenetic and phenotypic analyses

KW - Sylvatic DENV

UR - http://www.scopus.com/inward/record.url?scp=84855341199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855341199&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2011.11.018

DO - 10.1016/j.virol.2011.11.018

M3 - Article

C2 - 22178263

AN - SCOPUS:84855341199

SN - 0042-6822

VL - 423

SP - 58

EP - 67

JO - Virology

JF - Virology

IS - 1

ER -

Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this