TY - JOUR
T1 - Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome
AU - Lokugamage, Kumari
AU - Kariwa, Hiroaki
AU - Lokugamage, Nandadeva
AU - Miyamoto, Hironobu
AU - Iwasa, Masahiro
AU - Hagiya, Tomohiro
AU - Araki, Koichi
AU - Tachi, Atsushi
AU - Mizutani, Tetsuya
AU - Yoshimatsu, Kumiko
AU - Arikawa, Jiro
AU - Takashima, Ikuo
N1 - Funding Information:
We thank Dr. C. Hung and Dr. H. Wang of the Institute of Virology, Chinese Academy of Preventive Medicine, for providing the Chinese virus isolates and information regarding their characteristics. This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sport, Science, and Technology, Japan (projects 1357529 and 13660311), and by Health Science Grants for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health, Labor, and Welfare, Japan.
PY - 2004/5
Y1 - 2004/5
N2 - The genetic and antigenic characteristics of the Amur (AMR) and Far East (FE) virus lineages, which are both within the genus Hantavirus, were studied. Representative viruses, H5 and B78 for AMR and Bao 14 for FE, were used. The entire small (S) and medium (M) segments, except for the 3′- and 5′-ends, were sequenced. The deduced amino acid sequences of AMR had 96.7 and 92.0-92.2% identities with the Hantaan (HTN) virus in the S and M segments, respectively. The amino acid sequences of FE had 99.1 and 97.9% identities in the S and M segments, respectively. The three viral strains and HTN virus had similar binding patterns to a panel of monoclonal antibodies (MAbs), except that one MAb did not bind AMR. However, sera from Apodemus peninsulae, naturally infected with AMR virus, neutralized homologous viruses at 1:160 to 1:320 dilutions and HTN at 1:20 to 1:40 dilutions. The anti-AMR serum neutralized homologous viruses at a 1:80 dilution and HTN at a 1:40 dilution. The anti-HTN serum did not neutralize AMR (<1:40 dilution), although it had a high neutralizing titer (1:320) against the homologous virus. Therefore, we suggest that AMR virus may constitute a distinct serotype within the genus Hantavirus.
AB - The genetic and antigenic characteristics of the Amur (AMR) and Far East (FE) virus lineages, which are both within the genus Hantavirus, were studied. Representative viruses, H5 and B78 for AMR and Bao 14 for FE, were used. The entire small (S) and medium (M) segments, except for the 3′- and 5′-ends, were sequenced. The deduced amino acid sequences of AMR had 96.7 and 92.0-92.2% identities with the Hantaan (HTN) virus in the S and M segments, respectively. The amino acid sequences of FE had 99.1 and 97.9% identities in the S and M segments, respectively. The three viral strains and HTN virus had similar binding patterns to a panel of monoclonal antibodies (MAbs), except that one MAb did not bind AMR. However, sera from Apodemus peninsulae, naturally infected with AMR virus, neutralized homologous viruses at 1:160 to 1:320 dilutions and HTN at 1:20 to 1:40 dilutions. The anti-AMR serum neutralized homologous viruses at a 1:80 dilution and HTN at a 1:40 dilution. The anti-HTN serum did not neutralize AMR (<1:40 dilution), although it had a high neutralizing titer (1:320) against the homologous virus. Therefore, we suggest that AMR virus may constitute a distinct serotype within the genus Hantavirus.
KW - Amur
KW - Bunyaviridae
KW - Hantaan
KW - Hantavirus
KW - Hantavirus infections
KW - Hemorrhagic fever with renal syndrome
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U2 - 10.1016/j.virusres.2003.12.031
DO - 10.1016/j.virusres.2003.12.031
M3 - Article
C2 - 15041180
AN - SCOPUS:12144287111
SN - 0168-1702
VL - 101
SP - 127
EP - 134
JO - Virus Research
JF - Virus Research
IS - 2
ER -