Generation of monoclonal antibody against protein phosphatase 5

O. M. Malanchuk; G. V. Ovcharenko; I. O. Tykhonkova; O. S. Khoma; U. A. Chorna; M. Yu Rybak; S. S. Palchevskyy

doi:10.7124/bc.000811

Generation of monoclonal antibody against protein phosphatase 5

O. M. Malanchuk, G. V. Ovcharenko, I. O. Tykhonkova, O. S. Khoma, U. A. Chorna, M. Yu Rybak, S. S. Palchevskyy

Research output: Contribution to journal › Article › peer-review

Abstract

Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, we describe the development of a PP5 specific monoclonal antibody (mAb) and characterize its suitability for Western blotting and immunoprecipitation. Methods. Hybridoma technology has been used for monoclonal antibody generation. Immunization was carried out with recombinant mouse PP5 expressed in Escherichia coli as a GST-tagged fusion protein. Results. mAb against PP5 has been generated. Conclusions. Generated mAb specifically recognizes recombinant and endogenous mouse and rat PP5 and is suitable for Western blotting and immunoprecipitation. This mAb will be useful tool for investigations of PP5 physiological role.

Original language	English (US)
Pages (from-to)	136-142
Number of pages	7
Journal	Biopolymers and Cell
Volume	29
Issue number	2
DOIs	https://doi.org/10.7124/bc.000811
State	Published - 2013
Externally published	Yes

Keywords

Hybridoma technique
Monoclonal antibody
Protein phosphatase 5

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.7124/bc.000811

Cite this

@article{2a7b552dd1344e1ca95bb8c3018246e0,

title = "Generation of monoclonal antibody against protein phosphatase 5",

abstract = "Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, we describe the development of a PP5 specific monoclonal antibody (mAb) and characterize its suitability for Western blotting and immunoprecipitation. Methods. Hybridoma technology has been used for monoclonal antibody generation. Immunization was carried out with recombinant mouse PP5 expressed in Escherichia coli as a GST-tagged fusion protein. Results. mAb against PP5 has been generated. Conclusions. Generated mAb specifically recognizes recombinant and endogenous mouse and rat PP5 and is suitable for Western blotting and immunoprecipitation. This mAb will be useful tool for investigations of PP5 physiological role.",

keywords = "Hybridoma technique, Monoclonal antibody, Protein phosphatase 5",

author = "Malanchuk, {O. M.} and Ovcharenko, {G. V.} and Tykhonkova, {I. O.} and Khoma, {O. S.} and Chorna, {U. A.} and Rybak, {M. Yu} and Palchevskyy, {S. S.}",

note = "Publisher Copyright: {\textcopyright} Institute of Molecular Biology and Genetics, NAS of Ukraine, 2013.",

year = "2013",

doi = "10.7124/bc.000811",

language = "English (US)",

volume = "29",

pages = "136--142",

journal = "Biopolymers and Cell",

issn = "0233-7657",

publisher = "National Academy of Sciences of Ukraine",

number = "2",

}

TY - JOUR

T1 - Generation of monoclonal antibody against protein phosphatase 5

AU - Malanchuk, O. M.

AU - Ovcharenko, G. V.

AU - Tykhonkova, I. O.

AU - Khoma, O. S.

AU - Chorna, U. A.

AU - Rybak, M. Yu

AU - Palchevskyy, S. S.

N1 - Publisher Copyright: © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2013.

PY - 2013

Y1 - 2013

N2 - Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, we describe the development of a PP5 specific monoclonal antibody (mAb) and characterize its suitability for Western blotting and immunoprecipitation. Methods. Hybridoma technology has been used for monoclonal antibody generation. Immunization was carried out with recombinant mouse PP5 expressed in Escherichia coli as a GST-tagged fusion protein. Results. mAb against PP5 has been generated. Conclusions. Generated mAb specifically recognizes recombinant and endogenous mouse and rat PP5 and is suitable for Western blotting and immunoprecipitation. This mAb will be useful tool for investigations of PP5 physiological role.

AB - Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, we describe the development of a PP5 specific monoclonal antibody (mAb) and characterize its suitability for Western blotting and immunoprecipitation. Methods. Hybridoma technology has been used for monoclonal antibody generation. Immunization was carried out with recombinant mouse PP5 expressed in Escherichia coli as a GST-tagged fusion protein. Results. mAb against PP5 has been generated. Conclusions. Generated mAb specifically recognizes recombinant and endogenous mouse and rat PP5 and is suitable for Western blotting and immunoprecipitation. This mAb will be useful tool for investigations of PP5 physiological role.

KW - Hybridoma technique

KW - Monoclonal antibody

KW - Protein phosphatase 5

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U2 - 10.7124/bc.000811

DO - 10.7124/bc.000811

M3 - Article

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SN - 0233-7657

VL - 29

SP - 136

EP - 142

JO - Biopolymers and Cell

JF - Biopolymers and Cell

IS - 2

ER -

Generation of monoclonal antibody against protein phosphatase 5

Abstract

Keywords

ASJC Scopus subject areas

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