Generation of an infectious Negev virus cDNA clone

Rodion V. Gorchakov, Robert B. Tesh, Scott C. Weaver, Farooq Nasar

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.

Original languageEnglish (US)
Pages (from-to)2071-2074
Number of pages4
JournalJournal of General Virology
Issue numberPART 9
StatePublished - Sep 2014

ASJC Scopus subject areas

  • Virology


Dive into the research topics of 'Generation of an infectious Negev virus cDNA clone'. Together they form a unique fingerprint.

Cite this