TY - JOUR
T1 - Further investigations of mutagenic cholesterol preparations
AU - Ansari, G. A.S.
AU - Walker, R. D.
AU - Smart, V. B.
AU - Smith, L. L.
N1 - Funding Information:
Acknorv/edgemenrs-The Clark in conducting reported here is gratefully Ames. University of California. us with the Sa/monr//a rJp/rimrrrium study was financially supported the US Public Health Service.
PY - 1982
Y1 - 1982
N2 - The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.
AB - The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.
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U2 - 10.1016/S0278-6915(82)80006-9
DO - 10.1016/S0278-6915(82)80006-9
M3 - Article
C2 - 7040182
AN - SCOPUS:0019989378
SN - 0278-6915
VL - 20
SP - 35
EP - 41
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 1
ER -