Functional annotation of hypothetical proteins related to antibiotic resistance in pseudomonas aeruginosa pa01

Background: Pseudomonas aeruginosa is a Gram-negative bacteria that causes a large range of human infections such as lung infection (cystic fibrosis) and urinary tract infection. Even worse, antibiotic resistant bacteria have become a serious health care problem throughout the last decade, and there is a need for a clear approach to regu-late and prevent the spread of pseudomonas aeruginosa resistance. Methods: A complete analysis of Pseudomonas aeruginosa proteomics data showed that 25% of proteins are hypo-thetical proteins (HPs) whose function is not precisely defined. HP gene sequence analysis offers a framework for defining sequence-function relationships with a deeper understanding of organisms' molecular mechanisms at the system level. In the current research, we used the power of different bioinformatics tools to assign the potential roles for the HPs based on protein family association, amino acid function, motifs, and pathway analysis. Results: The current findings show that 30 HPs have well-defined functions and are classified as enzymes, DNA binding, periplasmic binding protein, transport, etc. Seven HPs showed virulence characteristics that is to be ex-pected to be essential for Pseudomonas aeruginosa and pathogenesis survival. Conclusions: This study's findings may encourage a better understanding of virulence mechanisms, drug resis-tance, pathogenesis, and drug discovery to treat Pseudomonas aeruginosa infections.