Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

Satish K. Srivastava; Ansari Naseem H.; Brown James H.; Mark Petrash J.

doi:10.1016/0304-4165(82)90171-4

Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

Satish K. Srivastava, Ansari Naseem H., Brown James H., Mark Petrash J.

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.

Original language	English (US)
Pages (from-to)	210-214
Number of pages	5
Journal	BBA - General Subjects
Volume	717
Issue number	2
DOIs	https://doi.org/10.1016/0304-4165(82)90171-4
State	Published - Aug 6 1982

Keywords

(Lens)
Aldose reductase
Sorbitol 6-phosphate
Sorbitol dehydrogenase
Sorbitol kinase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0304-4165(82)90171-4

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title = "Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase",

abstract = "Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.",

keywords = "(Lens), Aldose reductase, Sorbitol 6-phosphate, Sorbitol dehydrogenase, Sorbitol kinase",

author = "Srivastava, {Satish K.} and {Naseem H.}, Ansari and {James H.}, Brown and J., {Mark Petrash}",

note = "Funding Information: This investigation was supported by PHS grants EY 01677 and EY 02260, awarded by the National Eye Institute, DHHS. The excellent technical assistance of Fannie M. Ravenell is gratefully acknowledged.",

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doi = "10.1016/0304-4165(82)90171-4",

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T1 - Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

AU - Srivastava, Satish K.

AU - Naseem H., Ansari

AU - James H., Brown

AU - J., Mark Petrash

N1 - Funding Information: This investigation was supported by PHS grants EY 01677 and EY 02260, awarded by the National Eye Institute, DHHS. The excellent technical assistance of Fannie M. Ravenell is gratefully acknowledged.

PY - 1982/8/6

Y1 - 1982/8/6

N2 - Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.

AB - Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.

KW - (Lens)

KW - Aldose reductase

KW - Sorbitol 6-phosphate

KW - Sorbitol dehydrogenase

KW - Sorbitol kinase

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Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

Abstract

Keywords

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