Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study

Miles Berger, David M. Murdoch, Janet S. Staats, Cliburn Chan, Jake P. Thomas, Grant E. Garrigues, Jeffrey N. Browndyke, Mary Cooter, Quintin J. Quinones, Joseph P. Mathew, Kent J. Weinhold, Cindy L. Amundsen, Shahrukh Bengali, Brian E. Brigman, W. Michael Bullock, Jessica Carter, Joseph Chapman, Vanessa Cheong Yee Ching, Harvey J. Cohen, Brian ColinThomas A. D'Amico, Michael J. Devinney, James K. Deorio, Tressa Ellet, Ramon M. Esclamado, Michael N. Ferrandino, Jeffrey Gadsden, Jason Guercio, Ashraf Habib, David H. Harpole, Mathew G. Hartwig, Ehimemen Iboaya, Brant A. Inman, Anver Khan, Sandhya Lagoo-Deenadayalan, Paula S. Lee, Walter T. Lee, John Lemm, Howard Levinson, Christopher Mantyh, David L. McDonagh, John Migaly, Suhail K. Mithani, Eugene Moretti, Judd W. Moul, Mark F. Newman, Katherine Ni, Brian Ohlendorf, Alexander Perez, Andrew C. Peterson, Vikram Ponussamy, Glenn M. Preminger, Cary N. Robertson, Sanziana A. Roman, Scott Runyon, Aaron Sandler, Randall P. Scheri, S. Kendall Smith, Leonard Talbot, Julie K.M. Thacker, Betty C. Tong, Alexander Tu, Steven N. Vaslef, Nathan Waldron, Xueyuan Wang, Heather Whitson, Victoria Wickenheisser, Christopher Young

Research output: Contribution to journalArticlepeer-review


Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

Original languageEnglish (US)
Pages (from-to)E150-E154
JournalAnesthesia and analgesia
Issue number5
StatePublished - Nov 1 2019

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine


Dive into the research topics of 'Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study'. Together they form a unique fingerprint.

Cite this