Fibrin biomatrix-conjugated platelet-derived growth factor AB accelerates wound healing in severe thermal injury

Rainer Mittermayr, Ludwik Branski, Martina Moritz, Marc G. Jeschke, David N. Herndon, Daniel Traber, Jason Schense, Jörg Gampfer, Andreas Goppelt, Heinz Redl

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Controlled delivery of growth factors from biodegradable biomatrices could accelerate and improve impaired wound healing. The study aim was to determine whether platelet-derived growth factor AB (PDGF.AB) with a transglutaminase (TG) crosslinking substrate site released from a fibrin biomatrix improves wound healing in severe thermal injury. The binding and release kinetics of TG-PDGF.AB were determined in vitro. Third-degree contact burns (dorsum of Yorkshire pigs) underwent epifascial necrosectomy 24h post-burn. Wound sites were covered with autologous meshed (3:1) split-thickness skin autografts and either secured with staples or attached with sprayed fibrin sealant (FS; n=8/group). TG-PDGF.AB binds to the fibrin biomatrix using the TG activity of factor XIIIa, and is subsequently released through enzymatic cleavage. Three doses of TG-PDGF.AB in FS (100ng, 1μg and 11μg/ml FS) were tested. TG-PDGF.AB was bound to the fibrin biomatrix as evidenced by western blot analysis and subsequently released by enzymatic cleavage. A significantly accelerated and improved wound healing was achieved using sprayed FS containing TG-PDGF.AB compared to staples alone. Low concentrations (100ng-1μg TG-PDGF.AB/ml final FS clot) demonstrated to be sufficient to attain a nearly complete closure of mesh interstices 14days after grafting. TG-PDGF.AB incorporated in FS via a specific binding technology was shown to be effective in grafted third-degree burn wounds. The adhesive properties of the fibrin matrix in conjunction with the prolonged growth factor stimulus enabled by this binding technology could be favourable in many pathological situations associated with wound-healing disturbances.

Original languageEnglish (US)
Pages (from-to)E275-E285
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number5
StatePublished - May 1 2016


  • Binding technology
  • Fibrin biomatrix
  • PDGF
  • Thermal injury
  • Wound healing

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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