Fetal wound healing using a genetically modified murine model: the contribution of P-selectin

Bindi Naik-Mathuria, Andre N. Gay, Ling Yu, Jean E. Hsu, C. Wayne Smith, Oluyinka O. Olutoye

Research output: Contribution to journalArticlepeer-review


Purpose: During early gestation, fetal wounds heal with paucity of inflammation and absent scar formation. P-selectin is an adhesion molecule that is important for leukocyte recruitment to injury sites. We used a murine fetal wound healing model to study the specific contribution of P-selectin to scarless wound repair. Methods: Linear excisional wounds were created on the dorsa of E15.5 and E17.5 gestation fetuses in wild-type and P-selectin (-/-) mice (term = 19 days). Wounds were harvested at various time-points after wounding and analyzed using histology and immunohistochemistry. Results: The E15.5 wounds in both wild-type and P-selectin (-/-) fetuses healed scarlessly and with minimal inflammation, whereas E17.5 wounds healed with fibrosis and inflammation. However, the scars of the P-selectin (-/-) wounds appeared slightly different than wild-type. There were significantly more inflammatory cells in E17.5 wild-type wounds 6 hours after injury (P < .001), but the difference was no longer significant by 24 hours. Finally, reepithelialization was slower in the E15.5 knockout wounds compared to their wild-type counterparts. Conclusions: Absence of P-selectin delays inflammatory cell recruitment and reepithelialization of fetal wounds; however, scar formation still occurs in late gestation wounds. The contribution of specific molecules to fetal wound healing can be elucidated using murine knockout or transgenic models.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalJournal of Pediatric Surgery
Issue number4
StatePublished - Apr 2008
Externally publishedYes


  • Fetal wound healing
  • Knockout fetal wound healing model
  • Murine fetal wound healing model
  • P-selectin
  • Scarless wound healing

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health


Dive into the research topics of 'Fetal wound healing using a genetically modified murine model: the contribution of P-selectin'. Together they form a unique fingerprint.

Cite this