FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans

Sterling C.T. Martin, Hiroshi Qadota, Andres F. Oberhauser, Jeff Hardin, Guy M. Benian

Research output: Contribution to journalArticlepeer-review

Abstract

Protein phosphatase 2A (PP2A) functions in a variety of cellular contexts. PP2A can assemble into four different complexes based on the inclusion of different regulatory or targeting subunits. The B''' regulatory subunit "striatin" forms the STRIPAK complex consisting of striatin, a catalytic subunit (PP2AC), striatin-interacting protein 1 (STRIP1), and MOB family member 4 (MOB4). In yeast and Caenorhabditis elegans, STRIP1 is required for formation of the endoplasmic reticulum (ER). Because the sarcoplasmic reticulum (SR) is the highly organized muscle-specific version of ER, we sought to determine the function of the STRIPAK complex in muscle using C. elegans. CASH-1 (striatin) and FARL-11 (STRIP1/2) form a complex in vivo, and each protein is localized to SR. Missense mutations and single amino acid losses in farl-11 and cash-1 each result in similar sarcomere disorganization. A missense mutation in farl-11 shows no detectable FARL-11 protein by immunoblot, disruption of SR organization around M-lines, and altered levels of the SR Ca+2 release channel UNC-68.

Original languageEnglish (US)
Article number0083
JournalMolecular Biology of the Cell
Volume34
Issue number9
DOIs
StatePublished - Aug 1 2023

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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