TY - JOUR
T1 - Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages
AU - Li, Li
AU - Feng, Haimin
AU - Da, Qin
AU - Jiang, Honglin
AU - Chen, Lang
AU - Xie, Linlin
AU - Huang, Qiuling
AU - Xiong, Hairong
AU - Luo, Fan
AU - Kang, Lei
AU - Zeng, Yan
AU - Hu, Haitao
AU - Hou, Wei
AU - Feng, Yong
N1 - Publisher Copyright:
© 2016, Springer-Verlag Wien.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.
AB - A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.
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U2 - 10.1007/s00705-016-2755-5
DO - 10.1007/s00705-016-2755-5
M3 - Article
C2 - 26831929
AN - SCOPUS:84964262358
SN - 0304-8608
VL - 161
SP - 1115
EP - 1123
JO - Archives of virology
JF - Archives of virology
IS - 5
ER -