Expression of anchorin CII (cartilage annexin V) in human young, normal adult, and osteoarthritic cartilage

Jürgen Mollenhauer, Meng Tuck Mok, Karen B. King, Malini Gupta, Susan Chubinskaya, Holger Koepp, Ada A. Cole

Research output: Contribution to journalArticlepeer-review

Abstract

In its tissue-specific function as a collagen receptor of chondrocytes, cartilage annexin V (anchorin CII) occupies a key position in the organization of the cell-extracellular matrix (ECM) junction for the tissue. The general role of annexin V (Anx V) in other tissues suggests involvement in cellular secretory processes and in regulation of apoptosis. Immunohistochemical analysis of Anx V in growth plate cartilage, confirmed by in situ hybridization, suggests that Anx V is prominently expressed and forms a major constituent of growth plate chondrocytes. Anx V epitopes are also located in the pericellular matrix of hypertrophic cartilage. In adult articular cartilage the expression is downregulated, with the highest levels of immunostaining found in the upper third of the articular cartilage layers and almost no antigen found in the deep layers. Osteoarthritic (OA) cartilage is characterized by a significant upregulation of message and protein throughout the entire depth of the tissue, an accumulation of cytoplasmic annexin V epitopes, and a release of epitopes into the pericellular and interterritorial matrix, in part co-localized with granular structures. Therefore, Anx V expression and tissue distribution may serve as a histological marker for metabolic alterations and for changes in the cellular phenotype associated with OA.

Original languageEnglish (US)
Pages (from-to)209-220
Number of pages12
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number2
DOIs
StatePublished - Feb 1999
Externally publishedYes

Keywords

  • Annexin V
  • Articular cartilage
  • Growth plate
  • Immunohistochemistry
  • In situ hybridization

ASJC Scopus subject areas

  • Anatomy
  • Histology

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