Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

A. D.T. Barrett; A. Pryde; A. R. Medlen; T. N. Ledger; J. E. Whitby; C. A. Gibson; M. DeSilva; D. J. Groves; D. J. Langley; P. D. Minor

doi:10.1016/0264-410X(89)90196-5

Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

A. D.T. Barrett, A. Pryde, A. R. Medlen, T. N. Ledger, J. E. Whitby, C. A. Gibson, M. DeSilva, D. J. Groves, D. J. Langley, P. D. Minor

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

Original language	English (US)
Pages (from-to)	333-336
Number of pages	4
Journal	Vaccine
Volume	7
Issue number	4
DOIs	https://doi.org/10.1016/0264-410X(89)90196-5
State	Published - Aug 1989
Externally published	Yes

Keywords

Yellow fever
flavivirus
monoclonal antibodies

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0264-410X(89)90196-5

Cite this

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title = "Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies",

abstract = "Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.",

keywords = "Yellow fever, flavivirus, monoclonal antibodies",

author = "Barrett, {A. D.T.} and A. Pryde and Medlen, {A. R.} and Ledger, {T. N.} and Whitby, {J. E.} and Gibson, {C. A.} and M. DeSilva and Groves, {D. J.} and Langley, {D. J.} and Minor, {P. D.}",

year = "1989",

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T1 - Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

AU - Barrett, A. D.T.

AU - Pryde, A.

AU - Medlen, A. R.

AU - Ledger, T. N.

AU - Whitby, J. E.

AU - Gibson, C. A.

AU - DeSilva, M.

AU - Groves, D. J.

AU - Langley, D. J.

AU - Minor, P. D.

PY - 1989/8

Y1 - 1989/8

N2 - Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

AB - Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

KW - Yellow fever

KW - flavivirus

KW - monoclonal antibodies

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Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

Abstract

Keywords

ASJC Scopus subject areas

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