Evidence for different transport systems for oxidized glutathione and S-dinitrophenyl glutathione in human erythrocytes

E. F. LaBelle; S. V. Singh; S. K. Srivastava; Y. C. Awasthi

doi:10.1016/S0006-291X(86)80024-9

Evidence for different transport systems for oxidized glutathione and S-dinitrophenyl glutathione in human erythrocytes

E. F. LaBelle, S. V. Singh, S. K. Srivastava, Y. C. Awasthi

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of oxidized glutathione (GSSG) on the ATP-dependent transport of S-dinitrophenyl glutathione (Dnp-SG) by inside-out vesicles prepared from human erythrocytes and by intact erythrocytes has been studied. It is demonstrated that the transport of Dnp-SG is not inhibited by GSSG in either intact erythrocytes or in inside-out vesicles. These results suggest that Dnp-SG and GSSG are transported out of human erythrocytes by separate systems.

Original language	English (US)
Pages (from-to)	538-544
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80024-9
State	Published - Sep 16 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Evidence for different transport systems for oxidized glutathione and S-dinitrophenyl glutathione in human erythrocytes",

abstract = "The effect of oxidized glutathione (GSSG) on the ATP-dependent transport of S-dinitrophenyl glutathione (Dnp-SG) by inside-out vesicles prepared from human erythrocytes and by intact erythrocytes has been studied. It is demonstrated that the transport of Dnp-SG is not inhibited by GSSG in either intact erythrocytes or in inside-out vesicles. These results suggest that Dnp-SG and GSSG are transported out of human erythrocytes by separate systems.",

author = "LaBelle, {E. F.} and Singh, {S. V.} and Srivastava, {S. K.} and Awasthi, {Y. C.}",

note = "Funding Information: This investigation was supported by US PHS grants GM 32304, awarded by the National Institute of General Medical Sciences, EY 04396 and 01677, awarded by the National Eye Institute, and CA 27967, awarded by the NatioNal Cancer Institute. The authors wish to thank Geralyn Creadon and Leszek Wronski ~or technical assistance.",

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T1 - Evidence for different transport systems for oxidized glutathione and S-dinitrophenyl glutathione in human erythrocytes

AU - LaBelle, E. F.

AU - Singh, S. V.

AU - Srivastava, S. K.

AU - Awasthi, Y. C.

N1 - Funding Information: This investigation was supported by US PHS grants GM 32304, awarded by the National Institute of General Medical Sciences, EY 04396 and 01677, awarded by the National Eye Institute, and CA 27967, awarded by the NatioNal Cancer Institute. The authors wish to thank Geralyn Creadon and Leszek Wronski ~or technical assistance.

PY - 1986/9/16

Y1 - 1986/9/16

N2 - The effect of oxidized glutathione (GSSG) on the ATP-dependent transport of S-dinitrophenyl glutathione (Dnp-SG) by inside-out vesicles prepared from human erythrocytes and by intact erythrocytes has been studied. It is demonstrated that the transport of Dnp-SG is not inhibited by GSSG in either intact erythrocytes or in inside-out vesicles. These results suggest that Dnp-SG and GSSG are transported out of human erythrocytes by separate systems.

AB - The effect of oxidized glutathione (GSSG) on the ATP-dependent transport of S-dinitrophenyl glutathione (Dnp-SG) by inside-out vesicles prepared from human erythrocytes and by intact erythrocytes has been studied. It is demonstrated that the transport of Dnp-SG is not inhibited by GSSG in either intact erythrocytes or in inside-out vesicles. These results suggest that Dnp-SG and GSSG are transported out of human erythrocytes by separate systems.

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Evidence for different transport systems for oxidized glutathione and S-dinitrophenyl glutathione in human erythrocytes

Abstract

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