Evaluation of charging on macromolecules in electron cryomicroscopy

Jacob Brink; Michael B. Sherman; John Berriman; Wah Chiu

doi:10.1016/S0304-3991(97)00126-5

Evaluation of charging on macromolecules in electron cryomicroscopy

Jacob Brink, Michael B. Sherman, John Berriman, Wah Chiu

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

Original language	English (US)
Pages (from-to)	41-52
Number of pages	12
Journal	Ultramicroscopy
Volume	72
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3991(97)00126-5
State	Published - Apr 1 1998
Externally published	Yes

Keywords

Cryomicroscopy
Defocused diffraction
Electrical breakdown
Imaging charged samples
Secondary electron emission
Specimen current

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Instrumentation

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10.1016/S0304-3991(97)00126-5

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@article{969ba15becc44f9981f323f2d747231f,

title = "Evaluation of charging on macromolecules in electron cryomicroscopy",

abstract = "We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.",

keywords = "Cryomicroscopy, Defocused diffraction, Electrical breakdown, Imaging charged samples, Secondary electron emission, Specimen current",

author = "Jacob Brink and Sherman, {Michael B.} and John Berriman and Wah Chiu",

note = "Funding Information: This research was supported by grants from the National Center for Research Resources of National Institutes of Health (RR02250), the R. Welch Foundation (Q1242) and the W.M. Keck Foundation. We thank Dr. R.M. Glaeser for helpful discussions. J. Berriman would like to thank Dr. J. Cazaux for advice on excited insulators. Finally, we wish to thank Dr. P. Thuman-Commike and Mr. J.A. Lawton for critical reading of the manuscript. ",

year = "1998",

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doi = "10.1016/S0304-3991(97)00126-5",

language = "English (US)",

volume = "72",

pages = "41--52",

journal = "Ultramicroscopy",

issn = "0304-3991",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Evaluation of charging on macromolecules in electron cryomicroscopy

AU - Brink, Jacob

AU - Sherman, Michael B.

AU - Berriman, John

AU - Chiu, Wah

N1 - Funding Information: This research was supported by grants from the National Center for Research Resources of National Institutes of Health (RR02250), the R. Welch Foundation (Q1242) and the W.M. Keck Foundation. We thank Dr. R.M. Glaeser for helpful discussions. J. Berriman would like to thank Dr. J. Cazaux for advice on excited insulators. Finally, we wish to thank Dr. P. Thuman-Commike and Mr. J.A. Lawton for critical reading of the manuscript.

PY - 1998/4/1

Y1 - 1998/4/1

N2 - We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

AB - We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

KW - Cryomicroscopy

KW - Defocused diffraction

KW - Electrical breakdown

KW - Imaging charged samples

KW - Secondary electron emission

KW - Specimen current

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U2 - 10.1016/S0304-3991(97)00126-5

DO - 10.1016/S0304-3991(97)00126-5

M3 - Article

C2 - 9561796

AN - SCOPUS:0032053733

SN - 0304-3991

VL - 72

SP - 41

EP - 52

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 1-2

ER -

Evaluation of charging on macromolecules in electron cryomicroscopy

Abstract

Keywords

ASJC Scopus subject areas

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