Estimation and validation of gallic acid in polyherbal formulation by HPTLC

R. Mohan Kumar, K. Ilango, K. Ananth Kumar, N. Kasthuri Bai, G. P. Dubey, Aruna Agrawal

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Gallic acid is a phenyl propanoid, chemically it is 3, 4, 5 -Trihydroxy benzoic acid which is widely present in the plant kingdom and possesses activities like antioxidant, antimutagenic anticarcinogenic etc., In our current study we have carried out estimation and validation of gallic acid content in polyherbal formulation by HPTLC analysis which is very cheap and cost effective method. The samples were separated on pre-coated TLC plates with silica gel 60F254. The mobile phase used was Toluene: Methanol: Ethylacetate: Formic acid 30:5:55:10 (v/v/v/v). The plates were scanned at 280nm using CAMAG densitometer with WINCAT software. The LOD and LOQ for gallic acid were found to be 18ng and 55ng respectively. The developed method was validated as per ICH guide lines.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Issue numberSUPPL.4
StatePublished - 2013
Externally publishedYes


  • Gallic acid
  • Polyherbal formulation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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