Estimation and validation of gallic acid in polyherbal formulation by HPTLC

Gallic acid is a phenyl propanoid, chemically it is 3, 4, 5 -Trihydroxy benzoic acid which is widely present in the plant kingdom and possesses activities like antioxidant, antimutagenic anticarcinogenic etc., In our current study we have carried out estimation and validation of gallic acid content in polyherbal formulation by HPTLC analysis which is very cheap and cost effective method. The samples were separated on pre-coated TLC plates with silica gel 60F₂₅₄. The mobile phase used was Toluene: Methanol: Ethylacetate: Formic acid 30:5:55:10 (v/v/v/v). The plates were scanned at 280nm using CAMAG densitometer with WINCAT software. The LOD and LOQ for gallic acid were found to be 18ng and 55ng respectively. The developed method was validated as per ICH guide lines.