TY - JOUR
T1 - Escherichia coli isolated from a Crohn's disease patient adheres, invades, and induces inflammatory responses in polarized intestinal epithelial cells
AU - Eaves-Pyles, Tonyia
AU - Allen, Christopher A.
AU - Taormina, Joanna
AU - Swidsinski, Alexander
AU - Tutt, Christopher B.
AU - Eric Jezek, G.
AU - Islas-Islas, Martha
AU - Torres, Alfredo G.
N1 - Funding Information:
T.E.P. and this work were supported by a grant from the UTMB Gastrointestinal Research Interdisciplinary Program and T.E.P. and A.G.T. were funded by the UTMB John Sealy Memorial Endowment Fund for Biomedical Research. Fellowships from SURP, UTMB were awarded to M. I.-I. We want to thank Shilpa Patel for technical assistance. We would also like to thank Drs. Don Powell, Rick B. Pyles and Tor C. Savidge (UTMB) for their crucial comments and suggestions regarding the contents of this manuscript, Mardelle Susman (UTMB) for editorial and critical reading of the manuscript, and Dr. Jorge Blanco (Laboratorio de Referencia de E. coli [LREC], Lugo, Spain) for serotyping of the CD isolates.
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Inflammatory diseases of the intestinal tract are a major health concern both in the United States and around the world. Evidence now suggests that a new category of Escherichia coli, designated Adherent Invasive E. coli (AIEC) is highly prevalent in Crohn's Disease (CD) patients. AIEC strains have been shown to colonize and adhere to intestinal epithelial cells (IEC). However, the role AIEC strains play in the induction of an inflammatory response is not known. Therefore, we examined several E. coli strains (designated LF82, O83:H1, 6604 and 6655) that were isolated from CD patients for their ability to induce inflammation in two IEC, Caco-2BBe and T-84 cells. Results showed that each strain had varying abilities to adhere to and invade IEC as well as induced cytokine secretion from polarized IEC. However, E. coli O83:H1 displayed the best characteristics of AIEC strains as compared to the prototype AIEC strain LF82, inducing cytokine secretion from IEC and promoting immune cell migration through IEC. Upon further analysis, E. coli O83:H1 did not harbor virulence genes present in known pathogenic intestinal organisms. Further characterization of E. coli O83:H1 virulence determinants showed that a non-flagellated O83:H1 strain significantly decreased the organism's ability to adhere to and invade both IEC and elicit IEC cytokine secretion compared to the wild type and complemented strains. These findings demonstrate that E. coli O83:H1 possesses the characteristics of the AIEC LF82 strain that may contribute to the low-grade, chronic inflammation observed in Crohn's disease.
AB - Inflammatory diseases of the intestinal tract are a major health concern both in the United States and around the world. Evidence now suggests that a new category of Escherichia coli, designated Adherent Invasive E. coli (AIEC) is highly prevalent in Crohn's Disease (CD) patients. AIEC strains have been shown to colonize and adhere to intestinal epithelial cells (IEC). However, the role AIEC strains play in the induction of an inflammatory response is not known. Therefore, we examined several E. coli strains (designated LF82, O83:H1, 6604 and 6655) that were isolated from CD patients for their ability to induce inflammation in two IEC, Caco-2BBe and T-84 cells. Results showed that each strain had varying abilities to adhere to and invade IEC as well as induced cytokine secretion from polarized IEC. However, E. coli O83:H1 displayed the best characteristics of AIEC strains as compared to the prototype AIEC strain LF82, inducing cytokine secretion from IEC and promoting immune cell migration through IEC. Upon further analysis, E. coli O83:H1 did not harbor virulence genes present in known pathogenic intestinal organisms. Further characterization of E. coli O83:H1 virulence determinants showed that a non-flagellated O83:H1 strain significantly decreased the organism's ability to adhere to and invade both IEC and elicit IEC cytokine secretion compared to the wild type and complemented strains. These findings demonstrate that E. coli O83:H1 possesses the characteristics of the AIEC LF82 strain that may contribute to the low-grade, chronic inflammation observed in Crohn's disease.
KW - Bacteria
KW - Crohn's disease
KW - Immune cells
KW - Inflammation
KW - Intestine
UR - http://www.scopus.com/inward/record.url?scp=44349143335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44349143335&partnerID=8YFLogxK
U2 - 10.1016/j.ijmm.2007.05.011
DO - 10.1016/j.ijmm.2007.05.011
M3 - Article
C2 - 17900983
AN - SCOPUS:44349143335
SN - 1438-4221
VL - 298
SP - 397
EP - 409
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology
IS - 5-6
ER -