TY - JOUR
T1 - Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China
AU - Kariwa, Hiroki
AU - Zhong, Cui Bai
AU - Araki, Koichi
AU - Yoshimatsu, Kumiko
AU - Lokugamage, Kumari
AU - Lokugamage, Nandadeva
AU - Murphy, Michael E.
AU - Mizutani, Tetsuya
AU - Arikawa, Jiro
AU - Fukushima, Hiroshi
AU - Xiong, Hu
AU - Jiehua, Chen
AU - Takashima, Ikuo
PY - 2001
Y1 - 2001
N2 - Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China.
AB - Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China.
KW - Apodemus agrarius
KW - China
KW - Hantavirus
KW - Hemorrhagic fever with renal syndrome
KW - Rodent
UR - http://www.scopus.com/inward/record.url?scp=0035730513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035730513&partnerID=8YFLogxK
M3 - Article
C2 - 11590918
AN - SCOPUS:0035730513
SN - 0047-1917
VL - 49
SP - 105
EP - 114
JO - Japanese Journal of Veterinary Research
JF - Japanese Journal of Veterinary Research
IS - 2
ER -