Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant; Randall M. Goldblum; Richard Rahr; David O. Thueson; Jafar Farnam; James Gillaspy

doi:10.1016/0091-6749(81)90168-8

Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant, Randall M. Goldblum, Richard Rahr, David O. Thueson, Jafar Farnam, James Gillaspy

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

Original language	English (US)
Pages (from-to)	112-118
Number of pages	7
Journal	The Journal of Allergy and Clinical Immunology
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0091-6749(81)90168-8
State	Published - Aug 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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@article{51525e72d35a4522829ff25751e092df,

title = "Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms",

abstract = "IgG {"}blocking{"} antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.",

author = "Grant, {J. Andrew} and Goldblum, {Randall M.} and Richard Rahr and Thueson, {David O.} and Jafar Farnam and James Gillaspy",

note = "Funding Information: Supported in part by United States Public Health Service research grant AI-12621, General Clinical Research Program grant RR-73, Allergic Diseases Academic Award AI-00110 (Dr. Grant), Young Investigator Award AI-14879 (Dr. Thueson), the James W. McLaughlin Fellowship Fund (Dr. Famam), and Pharmacia Laboratories, Piscataway, N.J.",

year = "1981",

month = aug,

doi = "10.1016/0091-6749(81)90168-8",

language = "English (US)",

volume = "68",

pages = "112--118",

journal = "The Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

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number = "2",

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T1 - Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

AU - Grant, J. Andrew

AU - Goldblum, Randall M.

AU - Rahr, Richard

AU - Thueson, David O.

AU - Farnam, Jafar

AU - Gillaspy, James

N1 - Funding Information: Supported in part by United States Public Health Service research grant AI-12621, General Clinical Research Program grant RR-73, Allergic Diseases Academic Award AI-00110 (Dr. Grant), Young Investigator Award AI-14879 (Dr. Thueson), the James W. McLaughlin Fellowship Fund (Dr. Famam), and Pharmacia Laboratories, Piscataway, N.J.

PY - 1981/8

Y1 - 1981/8

N2 - IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

AB - IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

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Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

Abstract

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