TY - JOUR
T1 - Environmental regulation and colonization attributes of the long polar fimbriae (LPF) of Escherichia coli O157:H7
AU - Torres, Alfredo G.
AU - Milflores-Flores, Lorena
AU - Garcia-Gallegos, J. Gerardo
AU - Patel, Shilpa D.
AU - Best, Angus
AU - La Ragione, Roberto M.
AU - Martinez-Laguna, Ygnacio
AU - Woodward, Martin J.
N1 - Funding Information:
We want to thank Christopher B. Tutt for technical assistance and Christopher Allen and Gregory Whitlock for critical reading of the manuscript. This work was supported in part by institutional funds from the UTMB John Sealy Memorial Endowment Fund for Biomedical Research to A.G. Torres and from the Department for Environment, Food and Rural Affairs, United Kingdom (Project Grant OZO713) to M.J. Woodward. Fellowships from CONACYT and CECyT, Mexico and SURP, UTMB were awarded to L. Milfores-Flores.
PY - 2007/6/11
Y1 - 2007/6/11
N2 - The ability of Escherichia coli O157:H7 to colonize the intestinal epithelia is dependent on the expression of intimin and other adhesins. The chromosome of E. coli O157:H7 carries two loci encoding long polar fimbriae (LPF). These fimbriae mediate adherence to epithelial cells and are associated with colonization of the intestine. In order to increase our knowledge about the conditions controlling their expression and their role in colonization of an animal model, the environmental cues that promote expression of lpf genes and the role of E. coli O157:H7 LPF in intestinal colonization of lambs were investigated. We found that expression of lpf1 was regulated in response to growth phase, osmolarity, and pH; that lpf2 transcription was stimulated during late exponential growth and iron depletion; and that LPF impacts the ability of E. coli O157:H7 to persist in the intestine of infected 6-week-old lambs.
AB - The ability of Escherichia coli O157:H7 to colonize the intestinal epithelia is dependent on the expression of intimin and other adhesins. The chromosome of E. coli O157:H7 carries two loci encoding long polar fimbriae (LPF). These fimbriae mediate adherence to epithelial cells and are associated with colonization of the intestine. In order to increase our knowledge about the conditions controlling their expression and their role in colonization of an animal model, the environmental cues that promote expression of lpf genes and the role of E. coli O157:H7 LPF in intestinal colonization of lambs were investigated. We found that expression of lpf1 was regulated in response to growth phase, osmolarity, and pH; that lpf2 transcription was stimulated during late exponential growth and iron depletion; and that LPF impacts the ability of E. coli O157:H7 to persist in the intestine of infected 6-week-old lambs.
KW - E. coli O157:H7
KW - LP fimbriae
KW - Lamb model
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U2 - 10.1016/j.ijmm.2007.01.005
DO - 10.1016/j.ijmm.2007.01.005
M3 - Article
C2 - 17353147
AN - SCOPUS:34247575136
SN - 1438-4221
VL - 297
SP - 177
EP - 185
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology
IS - 3
ER -