Enterotoxin-associated DNA sequence homology between Salmonella species and Escherichia coli

A. K. Chopra; J. W. Peterson; C. W. Houston; R. Pericas; R. Prasad

doi:10.1111/j.1574-6968.1991.tb04336.x

Enterotoxin-associated DNA sequence homology between Salmonella species and Escherichia coli

A. K. Chopra, J. W. Peterson, C. W. Houston, R. Pericas, R. Prasad

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Multiple HindIII-restriction fragments of Salmonella typhimurium and Salmonella typhi chromosomal DNA exhibited homology with the heat-labile enterotoxin (LT1) gene of Escherichia coli as determined by Southern blot analysis. A 9.4 kb HindIII restriction fragment identified in S. typhimurium and S. typhi chromosomal DNA reacted with both eltA and eltB gene probes. However, the homology of the 9.4 kb DNA fragment from these Salmonella species was greater with eltB than eltA. In addition, a synthetic oligonucleotide probe, made to a portion of the putative G_M1-ganglioside binding region of cholera toxin (CT) and LT1, hybridized with the 9.4 kb DNA fragment of S. typhimurium but not with the 9.4 kb fragment found in S. typhi isolates. The hybridization of multiple restriction fragments of Salmonella DNA with eltA and eltB gene sequences further suggests duplication of the stx operon on the chromosome of these bacteria.

Original language	English (US)
Pages (from-to)	133-138
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	77
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04336.x
State	Published - Jan 15 1991

Keywords

Cholera toxin
Chromosomal DNA
G-Ganglioside
LT1 Enterotoxin
Southern blot hybridization
Synthetic oligonucleotide

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1991.tb04336.x

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title = "Enterotoxin-associated DNA sequence homology between Salmonella species and Escherichia coli",

abstract = "Multiple HindIII-restriction fragments of Salmonella typhimurium and Salmonella typhi chromosomal DNA exhibited homology with the heat-labile enterotoxin (LT1) gene of Escherichia coli as determined by Southern blot analysis. A 9.4 kb HindIII restriction fragment identified in S. typhimurium and S. typhi chromosomal DNA reacted with both eltA and eltB gene probes. However, the homology of the 9.4 kb DNA fragment from these Salmonella species was greater with eltB than eltA. In addition, a synthetic oligonucleotide probe, made to a portion of the putative GM1-ganglioside binding region of cholera toxin (CT) and LT1, hybridized with the 9.4 kb DNA fragment of S. typhimurium but not with the 9.4 kb fragment found in S. typhi isolates. The hybridization of multiple restriction fragments of Salmonella DNA with eltA and eltB gene sequences further suggests duplication of the stx operon on the chromosome of these bacteria.",

keywords = "Cholera toxin, Chromosomal DNA, G-Ganglioside, LT1 Enterotoxin, Southern blot hybridization, Synthetic oligonucleotide",

author = "Chopra, {A. K.} and Peterson, {J. W.} and Houston, {C. W.} and R. Pericas and R. Prasad",

note = "Funding Information: This work was supported by a Public Health Service research grant (5R01 AI-18401) from the National Institutes of Allergy and Infectious Diseases. We thank Dr. Steve Moseley, University of Washington and Children Hospital, Seattle, WA, for donating the plasmid pEWD299. The help of Elaine Young and Jacquelyn D. Lynch in the typing of the manuscript is fully acknowledged.",

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doi = "10.1111/j.1574-6968.1991.tb04336.x",

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pages = "133--138",

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AU - Peterson, J. W.

AU - Houston, C. W.

AU - Pericas, R.

AU - Prasad, R.

N1 - Funding Information: This work was supported by a Public Health Service research grant (5R01 AI-18401) from the National Institutes of Allergy and Infectious Diseases. We thank Dr. Steve Moseley, University of Washington and Children Hospital, Seattle, WA, for donating the plasmid pEWD299. The help of Elaine Young and Jacquelyn D. Lynch in the typing of the manuscript is fully acknowledged.

PY - 1991/1/15

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N2 - Multiple HindIII-restriction fragments of Salmonella typhimurium and Salmonella typhi chromosomal DNA exhibited homology with the heat-labile enterotoxin (LT1) gene of Escherichia coli as determined by Southern blot analysis. A 9.4 kb HindIII restriction fragment identified in S. typhimurium and S. typhi chromosomal DNA reacted with both eltA and eltB gene probes. However, the homology of the 9.4 kb DNA fragment from these Salmonella species was greater with eltB than eltA. In addition, a synthetic oligonucleotide probe, made to a portion of the putative GM1-ganglioside binding region of cholera toxin (CT) and LT1, hybridized with the 9.4 kb DNA fragment of S. typhimurium but not with the 9.4 kb fragment found in S. typhi isolates. The hybridization of multiple restriction fragments of Salmonella DNA with eltA and eltB gene sequences further suggests duplication of the stx operon on the chromosome of these bacteria.

AB - Multiple HindIII-restriction fragments of Salmonella typhimurium and Salmonella typhi chromosomal DNA exhibited homology with the heat-labile enterotoxin (LT1) gene of Escherichia coli as determined by Southern blot analysis. A 9.4 kb HindIII restriction fragment identified in S. typhimurium and S. typhi chromosomal DNA reacted with both eltA and eltB gene probes. However, the homology of the 9.4 kb DNA fragment from these Salmonella species was greater with eltB than eltA. In addition, a synthetic oligonucleotide probe, made to a portion of the putative GM1-ganglioside binding region of cholera toxin (CT) and LT1, hybridized with the 9.4 kb DNA fragment of S. typhimurium but not with the 9.4 kb fragment found in S. typhi isolates. The hybridization of multiple restriction fragments of Salmonella DNA with eltA and eltB gene sequences further suggests duplication of the stx operon on the chromosome of these bacteria.

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KW - Chromosomal DNA

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KW - LT1 Enterotoxin

KW - Southern blot hybridization

KW - Synthetic oligonucleotide

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Enterotoxin-associated DNA sequence homology between Salmonella species and Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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