TY - JOUR
T1 - Enterohemorrhagic Escherichia coli trivalent recombinant vaccine containing EspA, intimin and Stx2 induces strong humoral immune response and confers protection in mice
AU - Gu, Jiang
AU - Liu, Yanqing
AU - Yu, Shu
AU - Wang, Haiguang
AU - Wang, Qingxu
AU - Yi, Yong
AU - Zhu, Fengcai
AU - Yu, Xue jie
AU - Zou, Quanming
AU - Mao, Xuhu
N1 - Funding Information:
The authors are grateful to Dr. Donald Bouyer (Department of Pathology, Univ. of Texas Medical Branch) for review of the manuscript. This project was support by the National High Technology Research and Development Program of China (Grant No.: 2006AA02Z443, 2007AA02Z409) and the National Natural Science Foundation of China (Grant No.: 30770514, 30670107).
PY - 2009/9
Y1 - 2009/9
N2 - Enterohemorrhagic Escherichia coli (EHEC) is an important food-borne pathogen, which causes a wide spectrum of diseases ranging from hemorrhagic colitis to life-threatening hemolytic uremic syndrome (HUS). Currently, insufficient measures to prevent and treat EHEC infection make a vaccine against EHEC in great demand. EspA (E. coli secreted protein A), intimin, and Stx2 (Shiga toxin 2) are three predominant virulence factors of EHEC, and each of them has proved to be capable of inducing partial protective immunity. In this study, we constructed a trivalent recombinant protein designated EIS that is composed of EspA (E), C-terminal 300 amino acids of intimin (I) and B subunit of Stx2 (S), and tested it as vaccine using a mouse model. Our results showed that immunization of EIS induced strong humoral response to EspA, intimin and Stx2 and protected mice against the challenges with live EHEC or EHEC sonicated lysate. Moreover, it enhanced clearance of intestinally colonized bacteria. This work suggests that for EHEC vaccines using a combination of EspA, intimin and Stx2 antigens appears to be more effective than using any of these immunogens alone.
AB - Enterohemorrhagic Escherichia coli (EHEC) is an important food-borne pathogen, which causes a wide spectrum of diseases ranging from hemorrhagic colitis to life-threatening hemolytic uremic syndrome (HUS). Currently, insufficient measures to prevent and treat EHEC infection make a vaccine against EHEC in great demand. EspA (E. coli secreted protein A), intimin, and Stx2 (Shiga toxin 2) are three predominant virulence factors of EHEC, and each of them has proved to be capable of inducing partial protective immunity. In this study, we constructed a trivalent recombinant protein designated EIS that is composed of EspA (E), C-terminal 300 amino acids of intimin (I) and B subunit of Stx2 (S), and tested it as vaccine using a mouse model. Our results showed that immunization of EIS induced strong humoral response to EspA, intimin and Stx2 and protected mice against the challenges with live EHEC or EHEC sonicated lysate. Moreover, it enhanced clearance of intestinally colonized bacteria. This work suggests that for EHEC vaccines using a combination of EspA, intimin and Stx2 antigens appears to be more effective than using any of these immunogens alone.
KW - E. coli secreted protein A
KW - Enterohemorrhagic Escherichia coli
KW - Intimin
KW - Shiga toxin
KW - Trivalent subunit vaccine
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U2 - 10.1016/j.micinf.2009.04.024
DO - 10.1016/j.micinf.2009.04.024
M3 - Article
C2 - 19427397
AN - SCOPUS:68849089748
SN - 1286-4579
VL - 11
SP - 835
EP - 841
JO - Microbes and Infection
JF - Microbes and Infection
IS - 10-11
ER -