Enhancing the concordance of two commercial dengue IgG ELISAs by exchange of the calibrator sample

Tom Schüttoff, Awadalkareem Adam, Sven Reiche, Christian Jassoy

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Dengue IgG testing is being recommended before dengue vaccination. Presently, the diagnostic method of choice is the dengue IgG ELISA. Objective: Determine the test performance and concordance of two commercial dengue IgG ELISA kits. Study design: A diagnostic study to examine the sensitivity, specificity, accuracy and concordance of the Panbio Dengue Indirect IgG ELISA kit and the NovaLisa Dengue IgG ELISA kit. Sera (483) were from dengue-endemic regions in Sudan. Test performance characteristics were determined when tests were performed as indicated in the test kits and when the Panbio calibrator sample was used for both tests. Results: The sensitivity of the Panbio and the NovaLisa ELISA was 91.1% and 99.0% and the specificity was 79.4% and 50.9%. The Panbio test was slightly more accurate (87.5% compared with 84.0%). Quantitative measurement readings of the tests correlated. The calibrator samples gave different cutoff values. Replacing the NovaLisa cutoff sample with the Panbio calibrator sample raised the accuracy of the NovaLisa assay to 88% and increased the concordance of the tests from 82.8 to 93%. Conclusions: The study shows that the two dengue IgG ELISAs differed clearly in sensitivity and specificity and gave discordant results for 17.2% of the sera. For the most part the discrepancy depended on the calibrator sample. The findings indicate that an optimized dengue IgG calibrator standard can enhance accuracy and concordance of commercial dengue ELISAs. An optimized standard calibrator would make dengue IgG seroprevalence testing more reliable.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Clinical Virology
Volume118
DOIs
StatePublished - Sep 2019
Externally publishedYes

Keywords

  • Accuracy
  • Dengue
  • ELISA
  • IgG
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Enhancing the concordance of two commercial dengue IgG ELISAs by exchange of the calibrator sample'. Together they form a unique fingerprint.

Cite this