Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Madhubrata Ghosh; Loo Chien Wang; Roland G. Huber; Yunfeng Gao; Leslie K. Morgan; Nikhil Kumar Tulsian; Peter J. Bond; Linda J. Kenney; Ganesh S. Anand

doi:10.1016/j.str.2018.10.012

Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Madhubrata Ghosh, Loo Chien Wang, Roland G. Huber, Yunfeng Gao, Leslie K. Morgan, Nikhil Kumar Tulsian, Peter J. Bond, Linda J. Kenney, Ganesh S. Anand

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

Original language	English (US)
Pages (from-to)	302-314.e4
Journal	Structure
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/j.str.2018.10.012
State	Published - Feb 5 2019
Externally published	Yes

Keywords

EnvZ
H bonding
His-Asp/Glu dyad
amide hydrogen-deuterium exchange mass spectrometry
helix stabilization
histidine rotamerization
osmosensing
protein dynamics
two-component signal transduction

ASJC Scopus subject areas

Structural Biology
Molecular Biology

Access to Document

10.1016/j.str.2018.10.012

Cite this

@article{7ff77fc22d4642889223cf23e5d485dc,

title = "Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices",

abstract = "A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.",

keywords = "EnvZ, H bonding, His-Asp/Glu dyad, amide hydrogen-deuterium exchange mass spectrometry, helix stabilization, histidine rotamerization, osmosensing, protein dynamics, two-component signal transduction",

author = "Madhubrata Ghosh and Wang, {Loo Chien} and Huber, {Roland G.} and Yunfeng Gao and Morgan, {Leslie K.} and Tulsian, {Nikhil Kumar} and Bond, {Peter J.} and Kenney, {Linda J.} and Anand, {Ganesh S.}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2019",

month = feb,

day = "5",

doi = "10.1016/j.str.2018.10.012",

language = "English (US)",

volume = "27",

pages = "302--314.e4",

journal = "Structure",

issn = "0969-2126",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

AU - Ghosh, Madhubrata

AU - Wang, Loo Chien

AU - Huber, Roland G.

AU - Gao, Yunfeng

AU - Morgan, Leslie K.

AU - Tulsian, Nikhil Kumar

AU - Bond, Peter J.

AU - Kenney, Linda J.

AU - Anand, Ganesh S.

PY - 2019/2/5

Y1 - 2019/2/5

N2 - A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

AB - A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

KW - EnvZ

KW - H bonding

KW - His-Asp/Glu dyad

KW - amide hydrogen-deuterium exchange mass spectrometry

KW - helix stabilization

KW - histidine rotamerization

KW - osmosensing

KW - protein dynamics

KW - two-component signal transduction

UR - http://www.scopus.com/inward/record.url?scp=85060705702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060705702&partnerID=8YFLogxK

U2 - 10.1016/j.str.2018.10.012

DO - 10.1016/j.str.2018.10.012

M3 - Article

C2 - 30503779

AN - SCOPUS:85060705702

SN - 0969-2126

VL - 27

SP - 302-314.e4

JO - Structure

JF - Structure

IS - 2

ER -

Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this