@article{c43cc61fe1254cfda32373dda196a071,
title = "Emergence potential of sylvatic dengue virus type 4 in the urban transmission cycle is restrained by vaccination and homotypic immunity",
abstract = "Sylvatic dengue viruses (DENV) are both evolutionarily and ecologically distinct from human DENV and are maintained in an enzootic transmission cycle. Evidence of sylvatic human infections from West Africa and Southeast Asia suggests that sylvatic DENV come into regular contact with humans. Thus, this potential of emergence into the human transmission cycle could limit the potential for eradicating this cycle with vaccines currently in late stages of development. We assessed the likelihood of sylvatic DENV-4 emergence in the face of natural immunity to current human strains and vaccination with two DENV-4 vaccine candidates. Our data indicate homotypic neutralization of sylvatic and human DENV-4 strains by human primary convalescent and vaccinee sera but limited heterotypic immunity. These results suggest that emergence of sylvatic strains into the human cycle would be limited by homotypic immunity mediated by virus neutralizing antibodies produced by natural infection or vaccination.",
keywords = "Antigenic relationships, Dengue virus (DENV), Human DENV, Plaque reduction neutralization test (PRNT), Sylvatic DENV, Vaccine",
author = "Durbin, {Anna P.} and Mayer, {Sandra V.} and Rossi, {Shannan L.} and Amaya-Larios, {Irma Y.} and Jose Ramos-Castaneda and {Eong Ooi}, Eng and {Jane Cardosa}, M. and Munoz-Jordan, {Jorge L.} and Tesh, {Robert B.} and Messer, {William B.} and Weaver, {Scott C.} and Nikos Vasilakis",
note = "Funding Information: We thank Ilya Frolov for providing C6/36 cells. AP is supported by the National Institute of Allergy and Infectious Diseases Intramural Research Program, National Institutes of Health under contract HHSN272200900010C ; SVM is supported by the James W. McLaughlin Fellowship Fund ; SLR is supported by 2T32AI007536-11A1 postdoctoral training grant; EEO is supported by the Singapore National Research Foundation under its Translational Clinical Research Award administered by the National Medical Research Council; SW is supported for dengue research by NIH grant RO1 AI069145 ; WBM is supported by SERCEB/NIH U54 AI057157 SE-RP-004 and SE-CD-007 ; RT is supported by NIH contract HHSN272201000040I/HHSN27200004/D04 ; NV is supported by start-up funds provided by the Department of Pathology, UTMB . This work was presented in part at the {\textquoteleft}Emerging and Re-emerging Vector-Borne and Zoonotic Viral Infectious Diseases in Southeast Asia{\textquoteright}, meeting in Hanoi, Vietnam, September 2010 and at the 58th Annual Meeting of the American Society for Tropical Medicine and Hygiene, Atlanta, November 2010.",
year = "2013",
month = apr,
day = "25",
doi = "10.1016/j.virol.2013.01.018",
language = "English (US)",
volume = "439",
pages = "34--41",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",
}