Eilat virus induces both homologous and heterologous interference

Farooq Nasar, Jesse H. Erasmus, Andrew D. Haddow, Robert B. Tesh, Scott C. Weaver

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Most alphaviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, and humans. Occasionally, alphaviruses can spill over into the human population and cause disease characterized by debilitating arthralgia or fatal encephalitis. Recently, a unique alphavirus, Eilat virus (EILV), was described that readily infects mosquito but not vertebrate cell lines. Here, we investigated the ability of EILV to induce superinfection exclusion. Prior infection of C7/10 (. Aedes albopictus) cells with EILV induced homologous and heterologous interference, reducing the virus titers of heterologous superinfecting viruses (SINV, VEEV, EEEV, WEEV, and CHIKV) by ~10-10,000 fold and delaying replication kinetics by 12-48. h. Similar to in vitro infection, prior in vivo EILV infection of Aedes aegypti mosquitoes delayed dissemination of chikungunya virus for 3 days. This is the first evidence of heterologous interference induced by a mosquito-specific alphavirus in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
StatePublished - Oct 1 2015


  • Alphavirus
  • Eilat virus
  • Superinfection

ASJC Scopus subject areas

  • Virology


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