Efficient electrophoretic method to remove neutral additives from protein solutions followed by mass spectrometry analysis

Pei Jing Pai; Stephanie M. Cologna; William K. Russell; Gyula Vigh; David H. Russell

doi:10.1021/ac1029743

Efficient electrophoretic method to remove neutral additives from protein solutions followed by mass spectrometry analysis

Pei Jing Pai, Stephanie M. Cologna, William K. Russell, Gyula Vigh, David H. Russell

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A mass spectrometry (MS)-compatible, isoelectric point-based separation method for removal of neutral additives from protein solutions is described. The separation is performed by electrophoretic migration and trapping using a device referred to as membrane separated wells for isoelectric focusing and trapping (MSWIFT). Electrophoretic separation in the MSWIFT device is fast; the entire process can be carried out in a matter of minutes, and it does not require further sample cleanup prior to MS analysis. Proof-of-concept experiments in which neutral additives (e.g., Triton X-100, Tween 20, poly(ethylene glycol)) are removed from protein solutions using the MSWIFT device followed by MS analysis are described. Coupling the MSWIFT separation with ion mobility MS provides additional separation via the gas phase and assists in achieving higher quality ESI mass spectra when small amounts of additives remain in solution.

Original language	English (US)
Pages (from-to)	2814-2818
Number of pages	5
Journal	Analytical Chemistry
Volume	83
Issue number	7
DOIs	https://doi.org/10.1021/ac1029743
State	Published - Apr 1 2011
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac1029743

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title = "Efficient electrophoretic method to remove neutral additives from protein solutions followed by mass spectrometry analysis",

abstract = "A mass spectrometry (MS)-compatible, isoelectric point-based separation method for removal of neutral additives from protein solutions is described. The separation is performed by electrophoretic migration and trapping using a device referred to as membrane separated wells for isoelectric focusing and trapping (MSWIFT). Electrophoretic separation in the MSWIFT device is fast; the entire process can be carried out in a matter of minutes, and it does not require further sample cleanup prior to MS analysis. Proof-of-concept experiments in which neutral additives (e.g., Triton X-100, Tween 20, poly(ethylene glycol)) are removed from protein solutions using the MSWIFT device followed by MS analysis are described. Coupling the MSWIFT separation with ion mobility MS provides additional separation via the gas phase and assists in achieving higher quality ESI mass spectra when small amounts of additives remain in solution.",

author = "Pai, {Pei Jing} and Cologna, {Stephanie M.} and Russell, {William K.} and Gyula Vigh and Russell, {David H.}",

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T1 - Efficient electrophoretic method to remove neutral additives from protein solutions followed by mass spectrometry analysis

AU - Pai, Pei Jing

AU - Cologna, Stephanie M.

AU - Russell, William K.

AU - Vigh, Gyula

AU - Russell, David H.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - A mass spectrometry (MS)-compatible, isoelectric point-based separation method for removal of neutral additives from protein solutions is described. The separation is performed by electrophoretic migration and trapping using a device referred to as membrane separated wells for isoelectric focusing and trapping (MSWIFT). Electrophoretic separation in the MSWIFT device is fast; the entire process can be carried out in a matter of minutes, and it does not require further sample cleanup prior to MS analysis. Proof-of-concept experiments in which neutral additives (e.g., Triton X-100, Tween 20, poly(ethylene glycol)) are removed from protein solutions using the MSWIFT device followed by MS analysis are described. Coupling the MSWIFT separation with ion mobility MS provides additional separation via the gas phase and assists in achieving higher quality ESI mass spectra when small amounts of additives remain in solution.

AB - A mass spectrometry (MS)-compatible, isoelectric point-based separation method for removal of neutral additives from protein solutions is described. The separation is performed by electrophoretic migration and trapping using a device referred to as membrane separated wells for isoelectric focusing and trapping (MSWIFT). Electrophoretic separation in the MSWIFT device is fast; the entire process can be carried out in a matter of minutes, and it does not require further sample cleanup prior to MS analysis. Proof-of-concept experiments in which neutral additives (e.g., Triton X-100, Tween 20, poly(ethylene glycol)) are removed from protein solutions using the MSWIFT device followed by MS analysis are described. Coupling the MSWIFT separation with ion mobility MS provides additional separation via the gas phase and assists in achieving higher quality ESI mass spectra when small amounts of additives remain in solution.

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Efficient electrophoretic method to remove neutral additives from protein solutions followed by mass spectrometry analysis

Abstract

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