Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

Monica Longo; Venu Jain; Yuri P. Vedernikov; Robert E. Garfield; George R. Saade; Baha M. Sibai; Chin Chu Lin; Mark I. Evans; Dinesh M. Shah; Andrea G. Witlin; Longo

doi:10.1067/mob.2003.454

Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

Monica Longo, Venu Jain, Yuri P. Vedernikov, Robert E. Garfield, George R. Saade, Baha M. Sibai, Chin Chu Lin, Mark I. Evans, Dinesh M. Shah, Andrea G. Witlin, Longo

Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

Original language	English (US)
Pages (from-to)	1468-1476
Number of pages	9
Journal	American journal of obstetrics and gynecology
Volume	188
Issue number	6
DOIs	https://doi.org/10.1067/mob.2003.454
State	Published - Jun 1 2003

Keywords

Myometrium
Nitric oxide
Pregnant rat
Relaxin
Uterine artery

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1067/mob.2003.454

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@article{40cdd15192c84c99beef5f0cb0ed7789,

title = "Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro",

abstract = "OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.",

keywords = "Myometrium, Nitric oxide, Pregnant rat, Relaxin, Uterine artery",

author = "Monica Longo and Venu Jain and Vedernikov, {Yuri P.} and Garfield, {Robert E.} and Saade, {George R.} and Sibai, {Baha M.} and Lin, {Chin Chu} and Evans, {Mark I.} and Shah, {Dinesh M.} and Witlin, {Andrea G.} and Longo",

year = "2003",

month = jun,

day = "1",

doi = "10.1067/mob.2003.454",

language = "English (US)",

volume = "188",

pages = "1468--1476",

journal = "American journal of obstetrics and gynecology",

issn = "0002-9378",

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TY - JOUR

T1 - Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

AU - Longo, Monica

AU - Jain, Venu

AU - Vedernikov, Yuri P.

AU - Garfield, Robert E.

AU - Saade, George R.

AU - Sibai, Baha M.

AU - Lin, Chin Chu

AU - Evans, Mark I.

AU - Shah, Dinesh M.

AU - Witlin, Andrea G.

AU - Longo,

PY - 2003/6/1

Y1 - 2003/6/1

N2 - OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

AB - OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

KW - Myometrium

KW - Nitric oxide

KW - Pregnant rat

KW - Relaxin

KW - Uterine artery

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SP - 1468

EP - 1476

JO - American journal of obstetrics and gynecology

JF - American journal of obstetrics and gynecology

IS - 6

ER -

Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

Abstract

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