Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone

George R. Jackson, Deepa Sampath, Karin Werrbach-Perez, J. Regino Perez-Polo

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalBrain Research
Volume634
Issue number1
DOIs
StatePublished - Jan 14 1994
Externally publishedYes

Keywords

  • Catalase
  • Cell death
  • Glutathione peroxidase
  • HO
  • NGF
  • Oxidant resistance
  • PC12

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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