Effects of Bombesin on Growth of Human Small Cell Lung Carcinoma in Vivo

Robert W. Alexander, James R. Upp, Graeme J. Poston, Vicram Gupta, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Bombesin-like peptides are found in many different human tumors and are thought to function as an autocrine growth factor for small cell lung cancer in humans. In this study, a human small cell lung carcinoma (NCI-H69) was sc. implanted bilaterally into the flanks of 12 nude mice. The mice were randomized and divided into two groups and given either bombesin (20 μg/kg) or saline i.p. 3 times a day. Tumor areas were measured twice weekly for 6 wk. At sacrifice, the tumors and normal pancreas were excised, weighed, and assayed for DNA, RNA, and protein content. Significant stimulation of tumor growth was observed at weeks 4, 5, and 6. Tumor weight at sacrifice was significantly elevated (77%) above the control, as was DNA content (78%). Bombesin significantly increased the weight (42%), DNA (48%), and protein (61%) contents of the normal mouse pancreas. We conclude that bombesin may act as an autocrine growth factor, or indirectly through the release of other growth factors, on human small cell lung carcinoma.

Original languageEnglish (US)
Pages (from-to)1439-1441
Number of pages3
JournalCancer Research
Volume48
Issue number6
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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