Abstract
Results are reported on the cellular effects and the sensitivity of cultured tumor epithelial cells (TEC) derived from human ovarian cystadenocarcinoma and human umbilical vein-derived endothelial cells (HUVEC) to exogenous 5-aminolaevulinic acid (ALA) and ALA-induced photodynamic therapy (PDT). Cellular alterations and PDT efficiency were evaluated using colorimetric thiazolyl blue (MTT) assay, trypan blue exclusion assay, electron microscopy, and gel electrophoresis. ALA-induced protoporphyrin IX (PpIX) accumulation in TEC was associated with a concentration and time-dependent significant decrease in mitochondrial activity, increase in cell membrane permeability, and dark toxicity. Maximum PpIX loaded TEC demonstrated a high sensitivity to PDT. Neither cellular alterations nor PDT effects were observed in HUVEC under identical experimental conditions. These results indicate a potential clinical value for the use of ALA-mediated PDT to treat minimal residual disease in mucinous ovarian carcinoma. In addition, the ALA-induced PpIX cytotoxicity may be exported to a new chemotherapeutic regimen via a conventionally viewed photochemotherapeutic agent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8-20 |
| Number of pages | 13 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Volume | 64 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 1 2001 |
Keywords
- 5-aminolaevulinic acid
- Dark toxicity
- Human umbilical vein endothelial cells
- Ovarian cancer cells
- Photodynamic therapy
- Phototoxicity
- Protoporphyrin IX
ASJC Scopus subject areas
- Radiation
- Radiological and Ultrasound Technology
- Biophysics
- Radiology Nuclear Medicine and imaging